河南省济源市西瓜病毒检测及和西瓜花叶病毒基因组分析  

Detection of viruses infecting watermelon and genomic analysis of watermelon mosaic virus in Jiyuan of Henan Province

作　　者：贾宝华 李秀启 刘莉铭[2] 康保珊[2] 古勤生[2] 吴会杰[2] 彭斌[2] JIA Baohua;LI Xiuqi;LIU Liming;KANG Baoshan;GU Qinsheng;WU Huijie;PENG Bin(Jiyuan Academy of Agricultural Science,Jiyuan 459002,China;Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China)

机构地区：[1]济源市农业科学院,济源459002 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《植物病理学报》2024年第3期493-503,共11页Acta Phytopathologica Sinica

基　　金：财政部和农业农村部国家现代产业技术体系资助(CARS-25);中国农业科学院科技创新工程(CAASASTIP-2022-ZFRI-09);济源市科技攻关项目(20021012)。

摘　　要：本研究调查了2022年济源市3块西瓜种植地块西瓜病毒病害平均发病率约为97%,并收集20份疑似病毒病样品,包括西瓜14份、甜瓜4份、南瓜1份和杂草反枝苋(Amaranthaceae retroflexus L.)1份。选取3份西瓜和1份杂草反枝苋病样分别进行小RNA测序共检测出7种病毒。同时采用16种侵染西瓜的病毒特异性引物对所有的20份病样进行RT-PCR检测,与小RNA测序检测结果一致。西瓜花叶病毒(watermelon mosaic virus,WMV)和小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)是济源病毒病样中发生率前二的病毒,检出率分别是100%和85%。在一份反枝苋杂草样本中检测出WMV,表明反枝苋可能是WMV的中间寄主。从小RNA测序数据,获得了来自济源的4个WMV分离物的全长基因组。对WMV的全长基因组核苷酸序列进行一致性比较和系统进化分析,发现来自济源的WMV分离物具有较高的遗传多样性,表明该病毒在济源地区可能存在多个初始侵染源。本研究明确了济源地区西瓜病毒病种类,为该地区西瓜病毒病的防控策略制定及进一步的流行学研究奠定了理论基础。In 2022,a study investigating viral diseases in three watermelon planting plots was conducted in Jiyuan,revealing an average incidence rate of approximately 97%.Twenty samples,comprising 14 watermelon samples,4 melon samples,1 pumpkin sample and 1 pigweed(Amaranthaceae retroflexus L.),were collected from the field.Three watermelon samples and one Amaranthaceae weed sample underwent small RNA sequencing,separately,and virus detect identified seven viruses from the four small RNA sequencing data sets.Subsequently,RT-PCR using primer pairs specific for 16 watermelon-infecting viruses identified the same seven viruses in all 20 samples.Watermelon mosaic virus(WMV)and zucchini yellow mosaic virus(ZYMV)had detection rates of 100%and 85%,respectively,making them the two most prevalent viruses in watermelon fields.WMV was also identified in pigweed,which could serve as an intermediate host of WMV in the field.The full genome sequences of four WMV isolates were derived from four data sets of small RNA sequencing.Comparing the identity and conducting a phylogenetic analysis based on the full genome sequences of WMV revealed that WMV isolates from Jiyuan have very high genetic diversity,suggesting that there could be multiple initial infection sources of WMV in the region.This study identified the causal agents of viral diseases in watermelon in Jiyuan;established a theoretical foundation for developing prevention and control strategies and conducting epidemiological research on viral diseases in watermelon in the region.

关 键 词：西瓜 病毒病 小RNA测序 西瓜花叶病毒 系统进化 

分 类 号：S432.44[农业科学—植物病理学]