芒果炭疽病病原亚洲炭疽菌(Colletotrichum asianum)特异性PCR检测  

Specific PCR detection of Colletotrichum asianum causing mango anthracnose

作　　者：鲁萌萌 唐利华[1] 黄穗萍[1] 陈小林[1] 郭堂勋[1] 马立安[2] 陆建勋 李其利[1] LU Mengmeng;TANG Lihua;HUANG Suiping;CHEN Xiaolin;GUO Tangxun;MA Li'an;LU Jianxun;LI Qili(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China;College of Life Science,Yangtze University,Jingzhou 434025,China;Flowers Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,南宁530007 [2]长江大学生命科学学院,荆州434025 [3]广西壮族自治区农业科学院花卉研究所,南宁530007

出　　处：《植物病理学报》2024年第3期638-647,共10页Acta Phytopathologica Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-31);广西作物病虫害生物学重点实验室基金(20-065-30-KF-05)。

摘　　要：亚洲炭疽菌(Colletotrichum asianum)是一种能引发芒果炭疽病的优势病原菌。为建立一种能快速诊断该病原菌所引起的芒果炭疽病的PCR检测方法,本研究通过比较不同种类炭疽菌的全基因组DNA序列,获得了1条C.asianum特异性序列,设计并筛选得到1对特异性引物:caf1(5′-CCGTCAGACGGAATTATCAGC-3′)和car1(5′-CCGATCCTGTCTTTGAAATGG-3′)。结果表明,引物caf1/car1可以从C.asianum中扩增出长度为419 bp的特异性目标条带,灵敏度可达到1 pg·μL-1。利用该引物能够从人工接种C.asianum YN55-1的芒果叶片以及田间发生炭疽病的芒果叶片样品中快速稳定地检测出长度为419 bp的目标条带。本研究建立的特异性PCR检测方法为C.asianum导致的芒果炭疽病的田间监测和快速诊断提供了技术支持。Colletotrichum asianum is one of the most important pathogens that cause anthracnose on mango plants.To establish a method for rapid detection of C.asianum,a C.asianum-specific sequence was obtained by comparing genomic sequences of different Colletotrichum species,and a pair of specific primers:caf1(5'-CCGTCAGACGGAATTATCAGC-3')and car1(5'-CCGATCCTGTCTTTGAAATGG-3')was designed accor-dingly.Specificity test results showed that a 419-bp target amplicon could only be obtained from C.asianum rather than other Colletotrichum species and non-Colletotrichum fungal and bacterial species that we tested in this study using the primer pair caf1/car1,with a detection sensitivity of 1 pg·μL-1.The 419-bp amplicon could be detected from both mango leaves artificially inoculated with C.asianum strain YN55-1 and from naturally infected mango leaves with anthracnose symptoms.The specific PCR system developed in this study provides a technical support for monitoring and rapid diagnosis of mango anthracnose caused by C.asianum in the field.

关 键 词：芒果炭疽病 PCR检测 亚洲炭疽菌 

分 类 号：S432.1[农业科学—植物病理学]