金槐枝叶活性部位筛选及其化学成分研究  

Study on Active Fraction Screening and Chemical Constituents from the Stems and Leaves of Sophora Japonica Cv.Jinhuai

作　　者：邱显雯 符毓夏 潘争红 宁德生[2] 邹芷琪[3] Qiu Xianwen;Fu Yuxia;Pan Zhenghong;Ning Desheng;Zou Zhiqi(College of Pharmacy,Guilin Medical University,Guilin 541199,Chnia;Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China;College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]桂林医学院药学院,广西桂林541199 [2]广西壮族自治区中国科学院广西植物研究所广西植物功能物质与资源持续利用重点实验室,广西桂林541006 [3]广西中医药大学药学院,广西南宁530200

出　　处：《亚太传统医药》2024年第4期44-48,共5页Asia-Pacific Traditional Medicine

基　　金：广西创新驱动发展专项(桂科AA22096020-3)。

摘　　要：目的:筛选金槐枝叶的抗氧化和α-葡萄糖苷酶的抑制活性部位,研究其化学成分。方法:以α-葡萄糖苷酶抑制为筛选模型,并结合ABTS法、DPPH法对金槐枝叶不同极性部位进行活性评价,采用大孔树脂、C_(18)柱色谱和高效液相色谱等分离技术对活性部位进行分离纯化,根据波普数据分析以及文献比对鉴定化合物结构,并测定化合物对α-葡萄糖苷酶的抑制活性。结果:金槐枝叶乙酸乙酯部位的抗氧化和α-葡萄糖苷酶的抑制活性强于其他部位,并从中分离得到5个黄酮苷类化合物,分别鉴定为芦丁(1)、山柰酚3-O-芸香糖苷(2)、山柰酚3-O-洋槐糖苷(3)、Kaempferol 3-(2Grhamnosylrutinoside)(4)、Kaempferol 3-O-(2,6-di-α-L-rhamnopyranosyl)-β-D-galactopyranoside (5),且5个化合物均具有良好的α-葡萄糖苷酶抑制活性,IC_(50)分别为(79.92±3.24)μg/mL、(82.03±4.45)μg/mL、(89.21±3.87)μg/mL、(148.28±5.78)μg/mL、(152.36±4.96)μg/mL,化合物1~3的抑制活性高于阳性对照阿卡波糖。结论:乙酸乙酯部位是金槐枝叶的主要活性部位,且从中分离得到的化合物4和5为首次从该植物中分离得到。Objective:To screen the antioxidant and α-glucosidase inhibitory active fractions from the Stems and Leaves of Sophora japonica Cv.Jinhuai and study its chemical constituents.Methods:The activities of different fractions from the Stems and Leaves of Sophora japonica Cv.Jinhuai were evaluated by the α-glucosidase inhibition and the ABTS method and DPPH method The compounds were isolated from the active fractions by using the separation methods including macroporous resin,C_(18),and HPLC.Their chemical structures were identified according to spectra data analysis and literature comparison,and the inhibitory activity of compounds on α-glucosidase were determined.Results:The results showed that the antioxidative and α-glucosidase inhibitory activities of the ethyl acetate extract were stronger than others.Five flavonoid glycosides were obtained and their structures were identified as rutin(1),kaempferol-3-rutinoside(2),Kaempferol 3-O-robinobioside(3),Kaempferol 3-(2Grhamnosylrutinoside)(4),Kaempferol 3-O-(2,6-di-α-L-rhamnopyranosyl)-β-D-galactopyranoside(5),Compounds 4 and 5 were isolated from this plant for the first time.All five compounds exhibited good α-glucosidase inhibitory activities with the IC_(50) values of(79.92±3.24) μg/mL,(82.03±4.45)μg/mL,(89.21±3.87) μg/mL,(148.28±5.78)μg/mL and(152.36±4.96)μg/mL,respectively,which compounds 1~3 were significantly higher than the positive control of acarbose(140.45±4.88)μg/mL.Conclusion:The ethyl acetate extract was the main active extract of the Stems and Leaves of Sophora japonica Cv.Jinhuai and compounds 4 and 5 were isolated from the plant for the first time.

关 键 词：金槐 活性部位 抗氧化 Α-葡萄糖苷酶 化学成分 筛选 

分 类 号：R284.1[医药卫生—中药学]