宫颈细胞DNA倍体分析联合B7-H4和PKCδ对宫颈癌的诊断价值  

Diagnostic value of cervical cell DNA ploidy analysis combined with B7-H4 and PKCδfor cervical cancer

作　　者：张宁宁 杨哲 檀丽梅 李振宁 王迪 魏永志 Zhang Ningning;Yang Zhe;Tan Limei;Li Zhenning;Wang Di;Wei Yongzhi(Second Department of Gynecology,Shijiazhuang People's Hospital,Shijiazhuang 050000,China)

机构地区：[1]石家庄市人民医院妇二科,石家庄050000

出　　处：《国际肿瘤学杂志》2024年第5期286-291,共6页Journal of International Oncology

基　　金：2024年度河北省医学科学研究课题(20240725)。

摘　　要：目的探讨宫颈细胞DNA倍体分析联合负性共刺激分子B7同源物4(B7-H4)和蛋白激酶Cδ(PKCδ)对宫颈癌的诊断价值。方法选取2018年1月到2022年1月在石家庄市人民医院诊治的宫颈癌患者160例作为宫颈癌组,同期在本院做宫颈癌前筛查的女性160例作为对照组,根据检查结果分为正常或炎症组(n=52)、低级别宫颈上皮内瘤变(CIN)组(n=68)、高级别CIN组(n=40)。采用全自动细胞图像分析系统分析宫颈细胞DNA倍体情况。采用酶联免疫吸附法测定血清B7-H4、PKCδ水平。采用Pearson相关分析探讨血清B7-H4、PKCδ的相关性;采用受试者操作特征(ROC)曲线评估宫颈细胞DNA倍体分析联合血清B7-H4、PKCδ对宫颈癌的诊断价值;采用logistic多因素回归进行宫颈癌的危险因素分析。结果正常或炎症组、低级别CIN组、高级别CIN组、宫颈癌组DNA倍体阳性例数分别为16(30.8%)、27(39.7%)、26(65.0%)、127(79.4%),差异有统计学意义(H=55.86,P<0.001)。进一步两两比较发现,与正常或炎症组比较,高级别CIN组、宫颈癌组DNA倍体阳性比例均高(均P<0.05);与低级别CIN组比较,宫颈癌组DNA倍体阳性比例高(P<0.05)。正常或炎症组、低级别CIN组、高级别CIN组、宫颈癌组血清B7-H4水平分别为(57.21±10.21)、(79.17±11.34)、(92.73±15.36)、(126.56±20.25)ng/ml,差异有统计学意义(F=285.45,P<0.001),血清PKCδ水平分别为(89.34±18.29)、(71.79±15.82)、(53.39±11.84)、(40.23±10.21)ng/ml,差异有统计学意义(F=216.28,P<0.001),进一步两两比较发现,正常或炎症组、低级别CIN组、高级别CIN组、宫颈癌组血清B7-H4水平依次升高(均P<0.05);正常或炎症组、高级别CIN组、宫颈癌组血清PKCδ水平依次降低(均P<0.05)。Pearson检验分析结果显示,宫颈癌患者血清B7-H4、PKCδ水平呈负相关(r=-0.47,P<0.001)。ROC曲线分析显示,宫颈细胞DNA倍体诊断宫颈癌的曲线下面积(AUC)为0.82(95%CI为0.78~0.86),敏感性、特异性分别�Objective To investigate the diagnostic value of cervical cell DNA ploidy analysis combined with negative costimulatory molecule B7 homolog 4(B7-H4)and protein kinase Cδ(PKCδ)for cervical cancer.Methods A total of 160 cervical cancer patients diagnosed and treated at Shijiazhuang People's Hospital from January 2018 to January 2022 were selected as the cervical cancer group.Meantime,160 women who were screened for cervical cancer in our hospital during this period were selected as the control group.According to the examination results,they were divided into normal or inflammatory group(n=52),lowgrade cervical intraepithelial neoplasia(CIN)group(n=68)and high-grade CIN group(n=40).The automatic cell image analysis system was used to analyze the DNA ploidy of cervical cells.The levels of B7-H4 and PKCδin serum were determined by enzyme-linked immunosorbent assay.Pearson method was used to analyze the correlation between serum B7-H4 and PKCδ;the diagnostic value of cervical cell DNA ploidy analysis combined with serum B7-H4 and PKCδin cervical cancer was evaluated by the receiver operator characteristic(ROC)curve;multivariate logistic regression was used to analyze the risk factors of cervical cancer.Results The numbers of DNA ploidy positive cases of cervical cells in normal or inflammatory group,low-grade CIN group,high-grade CIN group and cervical cancer group were 16(30.8%),27(39.7%),26(65.0%)and 127(79.4%),respectively,with a statistically significant difference(H=55.86,P<0.001).Further pin-by-pair comparison showed that compared with normal or inflammatory groups,the proportion of DNA ploidy positive in high-grade CIN group and cervical cancer group were higher(both P<0.05).The proportion of DNA ploidy positive in cervical cancer group was higher than that in low-grade CIN group(P<0.05).Serum B7-H4 levels in normal or inflammatory group,low-grade CIN group,high-grade CIN group and cervical cancer group were(57.21±10.21),(79.17±11.34),(92.73±15.36),(126.56±20.25)ng/ml,respectively,with a statistically s

关 键 词：宫颈肿瘤 蛋白激酶Cδ 早期诊断 宫颈细胞DNA倍体 负性共刺激分子B7同源物4 

分 类 号：R737.33[医药卫生—肿瘤]