2023年7-10月河北省秦皇岛市新冠病毒基因组变异监测分析  

Monitoring and analysis of genome variation of SARS-CoV-2 in Qinhuangdao City,Hebei Province from July to October 2023

作　　者：文明新 于良 时晨[1] 王姝鑫 罗永松[1] WEN Mingxin;YU Liang;SHI Chen;WANG Shuxin;LUO Yongsong(Qinhuangdao Center for Disease Control and Prevention,Qinhuangdao,Hebei 066001,China)

机构地区：[1]秦皇岛市疾病预防控制中心,河北秦皇岛066001

出　　处：《中国国境卫生检疫杂志》2024年第3期305-307,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：秦皇岛市科技支撑计划项目(202301A055)。

摘　　要：目的实时监测秦皇岛市新冠病毒变异株流行趋势和变化特征,以及各亚型的构成,为制定本土防控政策提供依据。方法收集河北省秦皇岛市2023年7-10月本土新冠病毒感染者鼻咽拭子样本,每周5例,共80例,采用二代测序技术进行新冠病毒全基因组测序分析。结果经过Pangolin分型分析,80株本土新冠病毒中EG.5.1.1变异株52株,占65%;HK.3变异株13株,占16%;EG.5.1变异株5株,占6%;XBB.1.16变异株4株,占5%;还检出FY.3、FU.1、XBB.1.34、HK.1、GY.1、GY.6变异株,共6株,占7.50%。基因组变异分析显示:和EG.5.1.1变异株相比,HK.3变异株在刺突蛋白受体结合结构域(receptor binding domain,RBD)出现L455F和F456L两种氨基酸翻转突变。结论秦皇岛市2023年7-10月主要流行株为奥密克戎(Omicron)EG.5.1.1和HK.3两种变异株,在HK.3变异株刺突蛋白出现氨基酸F(苯丙氨酸)和L(亮氨酸)翻转。Objective To monitor the epidemic trend and variational characteristics of SARS-CoV-2 variant strains in Qinhuangdao city,as well as the composition of each subtype,and to provide evidence for formulating local prevention and control policies.Methods The pharyngeal swab samples of local COVID-19 infected patients were collected from July to October 2023 in Qinhuangdao city,Hebei Province,5 cases per week,a total of 80 cases.The whole genome of SARS-CoV-2 was analyzed by second-generation sequencing technology.Results The whole genome sequence of 80 cases of local SARS-CoV-2 infection was analyzed by Pangolin typing.There were 52 EG.5.1.1 variants,accounted for 65%,13 HK.3 variants,accounted for 16%,5 EG.5.1 variants,accounted for 6%,4 XBB.1.16 variants,accounted for 5%.FY.3,FU.1,XBB.1.34,HK.1,GY.1,GY.6 mutant strains were also detected,accounted for 7.50%.Genomic variation analysis showed that compared with EG.5.1.1 variant,HK.3 variant had two amino acid flipping mutations,L455F and F456L,in the receptor binding domain(RBD).Conclusion The main epidemic strains in Qinhuangdao city from July to October 2023 were Omicron EG.5.1.1 and HK.3 variants,and amino acids F(phenylalanine)and L(leucine)were flipped in the spike protein of HK.3 variants.

关 键 词：新冠病毒 全基因组测序 变异 刺突蛋白 二代测序 

分 类 号：R183.3[医药卫生—流行病学] R373.1[医药卫生—公共卫生与预防医学]