高脂饮食诱导的肥胖对下丘脑线粒体结构及功能的影响  

Effects of high fat diet induced obesity on the structure and function of hypothalamus mitochondria

作　　者：邢欣然 刘祺 杜勇 罗佳奇 高燕 苏泽礼[1] 王凯 XING Xinran;LIU Qi;DU Yong;LUO Jiaqi;GAO Yan;SU Zeli;WANG Kai(Department of Pediatric Surgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China;School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院小儿外科,宁夏银川750004 [2]宁夏医科大学临床医学院,宁夏银川750004

出　　处：《宁夏医学杂志》2024年第7期557-560,F0003,共5页Ningxia Medical Journal

基　　金：国家自然科学基金项目(82260308)。

摘　　要：目的研究高脂饮食诱导的肥胖对小鼠下丘脑线粒体结构及功能的影响。方法选取12只6周龄的C57BL/6J雄性小鼠,按照随机数字表法将其分为高脂饮食组(HFD)、正常饮食组(NC),分别给予高脂饮食及正常饮食12周后,通过测量体重、血脂、肝脏苏木素-伊红(HE)染色及油红O染色来判断肥胖模型是否构建成功;活性氧(ROS)检测试剂盒检测ROS含量,采用丙二醛(MDA)、超氧化物歧化酶(SOD)试剂盒检测MDA含量及SOD酶活性,透射电镜观察下丘脑线粒体的超微结构,采用线粒体膜电位(MMP)检测试剂盒(JC-1)检测MMP的变化,采用荧光素酶法检测三磷酸腺苷(ATP)含量。结果相较于NC组小鼠,HFD组小鼠的体重、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、血清中总胆固醇(TC)含量以及肝脏内的脂质沉积均明显增加(P<0.05),HFD组小鼠下丘脑SOD酶活性降低(P<0.05),而MDA含量升高(P<0.05),HFD组小鼠下丘脑线粒体水肿、线粒体嵴断裂,线粒体膜电位及ATP含量下降(P<0.05)。结论高脂饮食诱导的肥胖引起小鼠下丘脑线粒体结构紊乱及功能障碍。Objective To study the effect of obesity induced by high fat diet on the structure and function of hypothalamus mitochondria in mice.Methods Twelve male C57BL/6J mice,aged six weeks,were randomly assigned to two groups:a group fed with a high-fat diet(HFD)and a group fed with a normal diet(NC).After 12 weeks of being on their respective diets,various measurements such as body weight,blood lipid levels,Hematoxylin-Eosin staining(HE),and Oil-red O staining of the liver were conducted in order to assess the successful establishment of an obesity model.The presence of ROS was identified using a reactive oxygen species(ROS)detection kit,while the levels of MDA and SOD were measured using malondialdehyde(MDA)and superoxide dismutase(SOD)kits respectively.The transmission electron microscope was utilized to observe the ultrastructure of mitochondria in the hypothalamus,and the mitochondrial membrane potential(MMP)was assessed using an MMP(JC-1)detection kit.Additionally,luciferase enzyme was employed to measure ATP content.Results Compared with NC group,body weight,serum total cholesterol(TC),high density lipoprotein(HDL),low density lipoprotein(LDL)contents and liver lipid deposition of HFD group were significantly increased(P<0.05),hypothalamic SOD activity of HFD group was decreased(P<0.05),while MDA content was increased(P<0.05).HFD group mice hypothalamus mitochondrial edema,mitochondrial crest fracture,mitochondrial membrane potential and ATP content decreased(P<0.05).Conclusion Obesity induced by high-fat diet can cause structural disorder and dysfunction of hypothalamic mitochondria in mice.

关 键 词：高脂饮食 肥胖 下丘脑 线粒体 

分 类 号：R589.2[医药卫生—内分泌]