基于转录组挖掘香菇木质纤维素降解相关基因  

Transcriptomic Analysis of Genes Related to Lignocellulose Degradation in Lentinula edodes

作　　者：刘慧杰 游潇轩 戈永杰 尚晓冬[2] 谭琦[2] LIU Huijie;YOU Xiaoxuan;GE Yongjie;SHANG Xiaodong;TAN Qi(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201200,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)

机构地区：[1]上海海洋大学食品学院,上海201200 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2024年第4期33-45,共13页Acta Edulis Fungi

基　　金：上海市农业科学院卓越团队建设计划(沪农科卓[2022]001)。

摘　　要：对用木屑培养料(木屑组)和PDA培养基(PDA组)培养的香菇(Lentinula edodes)菌丝进行转录组测序分析,对两组样本进行主成分分析和相关性分析,对差异表达基因进行筛选和聚类分析;以PDA组为对照,对木屑组中显著差异表达的基因进行筛选以及GO功能富集、KEGG代谢通路分析;采用定量PCR方法验证9个差异表达基因表达情况。结果表明:培养基质造成的基因表达差异大于菌株,8个样本可分为两组(PDA组和木屑组);与PDA组相比,木屑组有677个基因的表达存在显著差异,其中445个基因(与木质纤维素降解相关的180个)上调,232个基因(与木质纤维素降解相关的38个)下调,表明木屑培养基能够显著诱导木质纤维素降解相关基因表达。GO功能富集分析结果表明,上调表达基因在氧化还原酶活性、水解酶活性、代谢过程、水解酶活性(水解O-糖基化合物)、水解酶活性(作用于糖基键)等代谢过程中显著富集。KEGG代谢通路分析结果表明,上调表达基因在溶酶体、转运蛋白、氨基糖和核苷酸糖代谢、精氨酸和脯氨酸代谢、淀粉和蔗糖代谢、肽酶和抑制剂通路中显著富集;下调表达基因在MAPK信号通路-酵母、不饱和脂肪酸生物合成、二噁英降解通路中显著富集。研究结果为揭示香菇培养基质利用的分子机制和选育高效利用基质菌株提供参考。In order to investigate the molecular mechanism underlying lignocellulose degradation in Lentinula edodes,hybrid progeny of L.edodes cultivars‘Shenxiang 215’and‘Huxiang F2’(divided into four groups)were cultured on both PDA medium(control)and sawdust medium.Then mycelia samples of different groups were sequenced for their transcriptiomes.Principle component analysis and correlation analysis were then used to screen and categorize transcriptome data for further analysis.Significantly differentially expressed genes between the PDA and sawdust cultured mycelia samples were identified,and then underwent GO functional enrichment and KEGG metabolic pathway analyses.Nine differentially expressed genes were further selected for qPCR verification.The results showed that culture medium brought about a greater difference than different strains,and the eight different groups of samples were categorized into two groups:PDA group(PA,PB,PC and PE)and sawdust group(MA,MB,MC and ME).Compared with the PDA group,there were 677 differentially expressed genes in the sawdust group,of which 445 genes were up-regulated and 232 genes were down-regulated.Among them,180 of the up-regulated genes and 38 of the down-regulated genes were involved in lignocellulose degradation,suggesting that sawdust significantly induced the expression of genes related to lignocellulose degradation in L.edodes.GO functional enrichment analysis showed that the up-regulated genes were primarily enriched in oxidoreductase activity,hydrolase activity,hydrolase activity(hydrolysis of O-glycosyl compounds),and hydrolase activity(acting on glycosyl bonds).KEGG pathway enrichment analysis showed that the up-regulated genes were significantly enriched in lysosomes,transporters,amino and nucleotide sugar metabolism,arginine and proline metabolism,starch sucrose metabolism,peptidases,and inhibitors.On the other hand,the down-regulated genes were significantly enriched in MAPK signaling-yeast,unsaturated fatty acid synthesis and dioxin degradation.These findings

关 键 词：香菇 转录组分析 木质纤维素 基质利用 

分 类 号：S646.12[农业科学—蔬菜学]