Inhibitory effect and mechanism of tannic acid against two starch digestive enzymes  

作　　者：钟毓秀 倪德让 杨玉波 李元一 王莉 田金虎 杨帆 叶兴乾 Yuxiu Zhong;Derang Ni;Yubo Yang;Yuanyi Li;Li Wang;Jinhu Tian;Fan Yang;Xingqian Ye(Department of Food Science and Nutrition,Zhejiang Key Laboratory for Agri-Food Processing,Fuli Institute of Food Science,Zhejiang R&D Centre for Food Technology and Equipment,Zhejiang University,Hangzhou,China;Institute of Science and Technology,Moutai Group,Zunyi,China;Key Laboratory of Industrial Microbial Resources Development,Kweichow Moutai Co.,Ltd.,Renhuai,China)

机构地区：[1]Department of Food Science and Nutrition,Zhejiang Key Laboratory for Agri-Food Processing,Fuli Institute of Food Science,Zhejiang R&D Centre for Food Technology and Equipment,Zhejiang University,Hangzhou,China [2]Institute of Science and Technology,Moutai Group,Zunyi,China [3]Key Laboratory of Industrial Microbial Resources Development,Kweichow Moutai Co.,Ltd.,Renhuai,China

出　　处：《Food Quality and Safety》2024年第1期115-123,共9页食品品质与安全研究（英文版）

基　　金：the Institute of Science and Technology,Moutai Group(No.MTGF2021020),China.

摘　　要：Backgrounds:Tannic acid(TA),as a plant-derived phenolic substance,is involved in regulating the activity of starch digestive enzymes,but its underlying mechanism remains unclear.Methods and Results:In the present study,inhibition rate and inhibition kinetics assays were performed and confrmed that TA had a strong inhibitory effect on bothα-amylase andα-glucosidase with IC50 values of 0.1585 mg/mL and 0.00542 mg/mL,respectively,through a mixed inhibition mode.The secondary structures of both enzymes were confrmed to be modifed by TA through circular dichroism(CD)spectra.Fluorescence quenching analysis revealed that the interaction between TA and two enzymes was a static process of pontaneous complex formation.Finally,molecular docking revealed that non-covalent bonds were the main interaction forces between TA and both enzymes.Conclusions:Thus,TA was a promising candidate for the inhibition of starch-digesting enzymes,and the present research provided insight into postprandial glucose regulation through polyphenols.

关 键 词：Tannic acid Α-AMYLASE Α-GLUCOSIDASE INHIBITION starch digestion. 

分 类 号：O62[理学—有机化学]