Effect of cinnamaldehyde on Rhizopus stolonifer and on the conservation of sweet potato  

作　　者：陈晨 薛智超 Penta Pristijono John B.Golding 陈刚 李永新 杨虎清 Chen Chen;Zhichao Xue;Penta Pristijono;John B.Golding;Gang Chen;Yongxin Li;Huqing Yang(College of Food and Health,Zhejiang A&F University,Hangzhou,China;Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,Zhejiang A&F University,Hangzhou,China;School of Environmental and Life Sciences,University of Newcastle,Ourimbah,NSW,Australia;NSW Department of Primary Industries,Ourimbah,NSW,Australia)

机构地区：[1]College of Food and Health,Zhejiang A&F University,Hangzhou,China [2]Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine,Zhejiang A&F University,Hangzhou,China [3]School of Environmental and Life Sciences,University of Newcastle,Ourimbah,NSW,Australia [4]NSW Department of Primary Industries,Ourimbah,NSW,Australia

出　　处：《Food Quality and Safety》2024年第1期212-225,共14页食品品质与安全研究（英文版）

基　　金：Scientifc Research Fund of Zhejiang Provincial Education Department(NO.Y202250109);the Key Projects of Ningbo Public Welfare Science and Technology Plan(No.202002N3081),China.

摘　　要：Objectives:Rhizopus stolonifer,which causes soft rot of sweet potato,has resulted in substantial loss of economy postharvest.Materials and Methods:The antifungal effect and mechanism of cinnamaldehyde(CIN)against R.stolonifer were explored by biochemical analysis and RNA-sequencing in this research,and the edible quality of CIN-treated sweet potato was evaluated.Results:The results showed that CIN inhibited the growth of R.stolonifer and reduced the incidence of soft rot in sweet potato at a minimum inhibitory concentration of 1000μL/L.The damage of the cell walls of R.stolonifer by CIN was associated with the upregulation of CHT1.Cytosolic leakage and malondialdehyde content increased following CIN-treatment,which was correlated with increased reactive oxygen species levels and decreased catalase activity.Upregulation of ERG genes and oxidative stress-related genes following CIN treatment was associated with impaired cell membrane integrity.The mitochondrial structure of R.stolonifer was damaged by CIN treatment resulting in decreased respiration rates and ATP production,which was correlated with lower expression levels of IDH1,COX4,and QCR7.Conclusions:Our research suggests that the inhibition of CIN on R.stolonifer was related to the disruption of its normal gene expression network,and CIN maintained the nutritional and edible quality of sweet potato.This study provides valuable insights into the antifungal mechanism of CIN on R.stolonifer and the maintenance of root quality,which contributes to the effcient use of CIN for controlling soft rot in sweet potato.

关 键 词：Ipomoea batatas soft rot CINNAMALDEHYDE antifungal mechanism TRANSCRIPTOME 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]