Ca^(2+)-independent ZmCPK2 is inhibited by Ca^(2+)-dependent ZmCPK17 during drought response in maize  

作　　者：Xiaoying Hu Jinkui Cheng Minmin Lu Tingting Fang Yujuan Zhu Zhen Li Xiqing Wang Yu Wang Yan Guo Shuhua Yang Zhizhong Gong 

机构地区：[1]State Key Laboratory of Plant Environmental Resilience,Frontiers Science Center for Molecular Design Breeding,Center for Crop Functional Genomics and Molecular Breeding,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]College of Life Sciences,Institute of Life Science and Green Development,Hebei University,Baoding 071002,China

出　　处：《Journal of Integrative Plant Biology》2024年第7期1313-1333,共21页植物学报（英文版）

基　　金：supported by grants from the National Science Foundation of China(32030008 and 31921001);the Beijing Outstanding University Discipline(2022YFF1001600).

摘　　要：Calcium oscillations are induced by different stresses.Calcium-dependent protein kinases(CDPKs/CPKs)are one major group of the plant calcium decoders that are involved in various processes including drought response.Some CPKs are calcium-independent.Here,we identified ZmCPK2 as a negative regulator of drought resistance by screening an overexpression transgenic maize pool.We found that ZmCPK2 does not bind calcium,and its activity is mainly inhibited during short term abscisic acid(ABA)treatment,and dynamically changed in prolonged treatment.Interestingly,ZmCPK2 interacts with and is inhibited by calcium-dependent ZmCPK17,a positive regulator of drought resistance,which is activated by ABA.ZmCPK17 could prevent the nuclear localization of ZmCPK2 through phosphorylation of ZmCPK2T60.ZmCPK2 interacts with and phosphorylates and activates ZmYAB15,a negative transcriptional factor for drought resistance.Our results suggest that drought stress-induced Ca^(2+)can be decoded directly by ZmCPK17 that inhibits ZmCPK2,thereby promoting plant adaptation to water deficit.

关 键 词：CDPK drought response PHOSPHORYLATION stomatal movement transcriptional regulation 

分 类 号：S513[农业科学—作物学]