絮凝技术在毕赤酵母发酵液固液分离中的应用研究  

Application of flocculation technology for solid-liquid separation of Pichia pastoris fermentation broth

作　　者：罗同阳 董聪 王玥 高庆华 贾振华[2] 王庆庆 刘小娜 LUO Tong-yang;DONG Cong;WANG Yue;GAO Qing-hua;JIA Zhen-hua;WANG Qing-qing;LIU Xiao-na

机构地区：[1]河北省微生物研究所有限公司,河北保定071051 [2]河北省科学院生物研究所,河北石家庄050081

出　　处：《饲料研究》2024年第12期77-81,共5页Feed Research

基　　金：河北省科学院高层次人才培养与资助项目(项目编号:2022G13);河北省科学院科技成果转化项目(项目编号:230202)。

摘　　要：试验旨在采用絮凝技术对毕赤酵母发酵液进行固液分离。以菌体絮凝率和酶活回收率为测定指标,采用单因素试验和正交试验,研究不同絮凝剂对重组毕赤酵母表达葡萄糖氧化酶发酵液絮凝效果的影响。结果显示,毕赤酵母发酵液最佳絮凝工艺为先加入2.0%聚合氯化铝,再加入1.0%硅藻土,最后加入0.2%阳离子型聚丙烯酰胺,且聚合氯化铝母液添加体积不超过发酵液总体积的20%。在此条件下,发酵液菌体絮凝率达90%以上,葡萄糖氧化酶的酶活回收率达85%以上。研究表明,采用絮凝技术能够对毕赤酵母发酵液进行固液分离,该方法有利于提高酶的生产效益,从而促进饲用酶制剂行业发展。The experiment aims to use flocculation technology for solid-liquid separation of the fermentation broth of Pichia pastoris.With the flocculated cell rate and enzyme activity recovery rate as the measurement indicators,singlefactor experiments and orthogonal experiments were conducted to study the effects of different flocculants on the flocculation of recombinant Pichia pastoris expressing glucose oxidase in the fermentation broth.The results showed that the optimal flocculation process for the Pichia pastoris fermentation broth was to first add 2.0%polyaluminum chloride,then 1.0%diatomite,and finally 0.2%cationic polyacrylamide,ensuring that the volume of polyaluminum chloride mother liquor added did not exceed 20%of the total volume of the fermentation broth.Under these conditions,the fermentation broth's cell flocculation rate reached over 90%,and the recovery rate of glucose oxidase enzyme activity reached over 85%.The study indicates that the use of flocculation technology can achieve solid-liquid separation of the Pichia pastoris fermentation broth,a method that is beneficial for improving the production efficiency of enzymes,thereby promoting the development of the feed enzyme preparation industry.

关 键 词：絮凝技术 毕赤酵母 固液分离 葡萄糖氧化酶 

分 类 号：S816[农业科学—饲料科学]