射干不同部位总三萜提取工艺优化及其抗氧化活性分析  

Optimization of total triterpene extraction process from different parts of Belamcanda chinensis and analysis of its antioxidant activity

作　　者：宫益霞 卢玉婷 任杰 刘翠娟[1] 谢炜康 耿一丁 GONG Yi-xia;LU Yu-ting;REN Jie;LIU Cui-juan;XIE Wei-kang;GENG Yi-ding

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《饲料研究》2024年第12期82-88,共7页Feed Research

基　　金：黑龙江省自然科学基金项目(项目编号:YQ2021H027);黑龙江省北药与功能食品特色学科项目(项目编号:HLJTSXK-2022-03);佳木斯大学国家自然科学基金培育项目(项目编号:JMSUGPZR2022-004)。

摘　　要：试验以射干为原材料,采用响应面法优化射干果皮总三萜(BCPT)和根茎总三萜(BCRT)提取工艺,分析其抗氧化活性。以温度、液料比、乙醇浓度、提取时间为主要影响因素,在单因素试验的基础上,以总三萜提取率为响应值,利用响应面试验优化BCPT和BCRT的提取条件。利用香草醛-冰乙酸-高氯酸法对BCPT、BCRT定量分析,并测定BCPT、BCRT对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)自由基的清除能力。结果显示:BCPT的最优提取工艺为提取温度70℃、液料比35 mL/g、乙醇浓度75%、提取时间210 min;BCRT的最优提取工艺为提取温度75℃、液料比35 mL/g、乙醇浓度70%、提取时间210 min。在最优条件下,BCPT、BCRT提取率分别为18.0772%、18.0743%。BCPT和BCRT对ABTS+自由基清除率分别为96.08%、94.32%;BCPT和BCRT对DPPH自由基的清除率为78.92%、58.01%。研究表明,试验建立的射干总三萜提取工艺简单合理,BCPT、BCRT均具有良好的抗氧化活性。The experiment used Belamcanda chinensis as raw material,and optimized the extraction process of total triterpenes from the fruit peel(BCPT)and the rhizome(BCRT)using the response surface methodology,and conducted an antioxidant activity analysis on them.The temperature,liquid to solid ratio,ethanol concentration,and extraction time were taken as the main influencing factors.Based on the single-factor experiments,the response value was the total triterpenes extraction rate,and the extraction conditions for BCPT and BCRT were optimized using the response surface experiments.The quantitative analysis of BCPT and BCRT was conducted using the vanillin-acetic acid-perchloric acid method,and the ability of BCPT and BCRT to scavenge DPPH and ABTS+radicals was measured.The results showed that the optimal extraction process for BCPT was a temperature of 70℃,a liquid to solid ratio of 35 mL/g,an ethanol concentration of 75%,and an extraction time of 210 minutes.The optimal extraction process for BCRT was a temperature of 75℃,a liquid to solid ratio of 35 mL/g,an ethanol concentration of 70%,and an extraction time of 210 minutes.Under the optimal conditions,the extraction rates of BCPT and BCRT were 18.0772%and 18.0743%,respectively.The scavenging rates of ABTS+radicals by BCPT and BCRT were 96.08%and 94.32%,respectively.The scavenging rates of DPPH radicals by BCPT and BCRT were 78.92%and 58.01%,respectively.The study indicates that the extraction process established in the experiment is simple and reasonable,and both BCPT and BCRT have good antioxidant activity.

关 键 词：射干 总三萜 工艺优化 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]