Allosteric DNAzyme-based encoder for molecular information transfer  

作　　者：Minghao Hu Tianci Xie Yuqiang Hu Longjie Li Ting Wang Tongbo Wu 

机构地区：[1]School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [2]Department of Oncology,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China [3]GI Cancer Research Institute,Tongji Hospital,Huazhong University of Science and Technology,Wuhan 430030,China [4]School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China [5]State Key Laboratory of Bioelectronics,National Demonstration Center for Experimental Biomedical Engineering Education,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China

出　　处：《Chinese Chemical Letters》2024年第7期235-241,共7页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.82172372);the Opening Research Fund of State Key Laboratory of Digital Medical Engineering(No.2023-M04)。

摘　　要：Dynamic DNA nanotechnology plays a significant role in nanomedicine and information science due to its high programmability based on Watson-Crick base pairing and nanoscale dimensions.Intelligent DNA machines and networks have been widely used in various fields,including molecular imaging,biosensors,drug delivery,information processing,and logic operations.Encoders serve as crucial components for information compilation and transfer,allowing the conversion of information from diverse application scenarios into a format recognized and applied by DNA circuits.However,there are only a few encoder designs with DNA outputs.Moreover,the molecular priority encoder is hardly designed.In this study,we introduce allosteric DNAzyme-based encoders for information transfer.The design of the allosteric domain and the recognition arm allows the input and output to be independent of each other and freely programmable.The pre-packaged mode design achieves uniformity of baseline dynamics and dynamics controllability.We also integrated non-nucleic acid molecules into the encoder through the aptamer design of the allosteric domain.Furthermore,we developed the 2^(n)-n encoder and the EndoⅣ-assisted priority encoder inspired by immunoglobulin's molecular structure and effector patterns.To our knowledge,the proposed encoder is the first enzyme-free DNA encoder with DNA output,and the priority encoder is the first molecular priority encoder in the DNA reaction network.Our encoders avoid complex operations on a single molecule,and their simple structure facilitates their application in complex DNA circuits and biological scenarios.

关 键 词：DNAZYME ENCODER Nucleic acids DNA circuit DNA strand displacement Dynamic DNA nanotechnology 

分 类 号：Q811.4[生物学—生物工程] TB383.1[一般工业技术—材料科学与工程]