侵染中国河北大豆的大豆花叶病毒的鉴定和全基因组序列分析  

Identification and complete genome sequence analysis of soybean mosaic virus from soybean in Hebei,China

作　　者：杨菲[1,2] 张明振[1] 周雪平 李方方[1] YANG Fei;ZHANG Mingzhen;ZHOU Xueping;LI Fangfang(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Key Laboratory of Integrate Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs,IPM Innovation Center of Hebei Province,International Science and Technology Joint Research Center on IPM of Hebei Province,Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京100193 [2]河北省农林科学院植物保护研究所,农业农村部华北北部作物有害生物综合治理重点实验室,河北省农业有害生物综合防治技术创新中心,河北省作物有害生物综合防治国际科技联合研究中心,保定071000

出　　处：《植物保护》2024年第4期39-47,共9页Plant Protection

基　　金：国家自然科学基金(31972244,32172385)。

摘　　要：本研究利用小RNA深度测序技术在河北卢龙大豆叶片上检测到6株大豆花叶病毒(soybean mosaic virus,SMV),命名为SMV-Gm1~SMV-Gm6。根据小RNA深度测序结果和参考基因组序列设计引物克隆了SMV河北分离物的基因组序列。测序结果经拼接后获得了6个SMV基因组全长序列,大小分别为9588 nt(SMV-Gm1、SMV-Gm3和SMV-Gm5)和9584 nt(SMV-Gm2、SMV-Gm4和SMV-Gm6)。开放阅读框位于基因组第132位至第9332位核苷酸,编码一个多聚蛋白(分子量约为350 kD)。BLAST比对和系统发育分析发现,SMV-Gm1、SMV-Gm3和SMV-Gm5与江苏SMV分离物(登录号:MH919386)的基因组核苷酸序列相似性最高,为98.06%~98.07%,且遗传距离较近,并与江苏、浙江和山西SMV分离物聚为一小簇;SMV-Gm2、SMV-Gm4和SMV-Gm6与韩国SMV分离物(登录号:FJ640954)的基因组核苷酸序列相似性最高,为98.48%~98.51%,且遗传距离较近。In this study,six isolates of soybean mosaic virus(labelled SMV-Gm1 to SMV-Gm6)were detected from soybean in Lulong,Hebei province using small RNA deep sequencing technology.Based on the sequencing results and a reference genome sequence,specific primers were designed to clone the genome sequences of these SMV isolates.Following assembly,six full-length SMV genome sequences were obtained,with sizes of 9588 nt for SMV-Gm1,SMV-Gm3,and SMV-Gm5,and 9584 nt for SMV-Gm2,SMV-Gm4,and SMV-Gm6.The open reading frame is located between 132nd to 9332nd nucleotides in each genome,encoding a polyprotein with a molecular weight of approximately 350 kD.BLAST alignment and phylogenetic analysis showed that SMV-Gm1,SMV-Gm3,and SMV-Gm5 showed the highest sequence similarity(98.06%to 98.07%)and relatively close genetic distance with the SMV isolate from Jiangsu province(accession number:MH919386),clustering with SMV isolates from Jiangsu,Zhejiang and Shanxi provinces.Similarly,the nucleotide sequences of SMV-Gm2,SMV-Gm4,and SMV-Gm6 exhibited the highest similarity(98.48%to 98.51%)with the SMV isolate from South Korea(accession number:FJ640954),indicating a close genetic relationship.

关 键 词：大豆花叶病毒 小RNA深度测序 基因组序列 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]