马铃薯块茎蛾取食诱导的马铃薯防御反应  

Defense response of potato to Phthorimaea operculella feeding

作　　者：祝春月 李智 矣晓翠 刘一依 姚遥 陈斌[2] 肖关丽[1] ZHU Chunyue;LI Zhi;YI Xiaocui;LIU Yiyi;YAO Yao;CHEN Bin;XIAO Guanli(College of Agriculture&Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南农业大学植物保护学院,昆明650201

出　　处：《植物保护》2024年第4期221-229,共9页Plant Protection

基　　金：国家自然科学基金(32360711,31760519);云南省教育厅科研基金(2023Y1026)。

摘　　要：为探究马铃薯在应答马铃薯块茎蛾Phthorimaea operculella取食时相关抗虫指标的动态变化,本研究以‘青薯9号’‘合作88’‘米拉’3个马铃薯品种为材料进行室内盆栽试验,在马铃薯块茎蛾取食0、3、6、12、24、48、72、96 h时取样测定各品种生理指标。结果表明,被马铃薯块茎蛾取食的叶片中过氧化氢酶、多酚氧化酶、萜烯合成酶活性,单宁和类黄酮含量与对照相比都有不同程度的显著升高,总氨基酸含量显著下降,且各指标在马铃薯品种之间存在差异。从各指标平均增降率来看,‘合作88’对马铃薯块茎蛾取食的响应程度要强于‘青薯9号’和‘米拉’‘米拉’的响应程度最小。本研究为进一步明确马铃薯抗虫生理防御机制和害虫综合防治提供理论依据。To investigate the dynamic changes of resistance-related indicators of potatoes in response to the feeding of potato tuber moth,Phthorimaea operculella Zeller,an indoor potting test was conducted using three potato varieties(‘Qingshu no.9’‘Hezuo 88’‘Mila’),the physiological resistance-related indices of potato eaten by P.operculella for 0,3,6,12,24,48,72 h,and 96 h were determined for each variety.The results showed that the activities of catalase(CAT),polyphenol oxidase(PPO),terpene synthase(TPS),the content of tannin,and flavonoids in the leaves of potatoes eaten by P.operculella were significantly increased compared to the control group.Conversely,the total amino acid content was significantly decreased,and there are variations in each index among potato varieties.Analysis of the rate of change for each index indicated that‘Hezuo 88’exhibited a stronger response to P.operculella feeding compared to‘Qingshu no.9’and‘Mila’,while‘Mila’displayed the least response.This study provides a theoretical understanding of potato’s physiological defense mechanisms against pests and supports the development of comprehensive pest control strategies.

关 键 词：马铃薯块茎蛾 马铃薯 防御酶 次生代谢物 营养物质 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]