聚多巴胺包覆硅化胶原的光热及抗菌性能研究  

A study on photothermal and antibacterial properties of silicified collagen coated with polydopamine

作　　者：张江山 雷晨 伏雨桐 马雨轩 慕昭 陈吉华 ZHANG Jiangshan;LEI Chen;FU Yutong;MA Yuxuan;MU Zhao;CHEN Jihua(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Department of Prosthodontics,The Third Hospital Affiliated of Air Force Military Medical University,Xi'an 710032,China)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第三附属医院口腔修复科,西安710032

出　　处：《实用口腔医学杂志》2024年第4期461-466,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:22205257,2022YFC2405900,2022YFC2405901)。

摘　　要：目的:通过多巴胺聚合作用构建聚多巴胺包覆硅化胶原(SC@PDA),探究该材料光热杀菌作用与生物安全性。方法:通过聚合反应将多巴胺包覆在硅化胶原表面构建SC@PDA。用扫描电镜、透射电镜观察SC@PDA表面形貌,硅酸释放实验检测材料硅酸缓释情况,808 nm激光照射与红外热像仪检测材料光热性能。CCK-8实验测试材料对大鼠骨髓间充质干细胞的毒性,急性全身毒性实验评价材料对大鼠的生物安全性。以SD大鼠为研究模型进行抗菌性能测试。将材料分别与金黄色葡萄球菌及大肠杆菌共培养6 h后,用808 nm激光(1.5 W/cm^(2))照射10 min,之后继续培养6 h,用吸光度检测计算材料光热抑菌率。在大鼠股骨缺损感染模型中观察材料植入缺损部位7 d后材料的抗感染效果。结果:SC@PDA具有良好的光热性能,保持了硅化胶原的硅酸缓释性能,无细胞毒性,无急性全身毒性。体内外抗菌实验表明,SC@PDA具有良好的光热抗菌能力。结论:SC@PDA具有优良的生物相容性,同时多巴胺包覆赋予了硅化胶原支架优异的光热抗菌性能。Objective:To construct polydopamine-coated silicified collagen(SC@PDA)by polymerization of dopamine,and to explore its biological safety and photothermal antibacterial properties.Methods:Dopamine was coated on the surface of silicified collagen by polymerization reaction to construct [e-mail] surface morphology of SC@PDA was observed by SEM and TEM,the silicic acid release was detected by silicic acid release experiment,and the photothermal properties of SC@PDA were detected by 808 nm laser irradiation and infrared thermography.The biocompatibility of SC@PDA was evaluated by CCK-8 assay with rat bone marrow mesenchymal stem cells and acute systemic toxicity test in SD rats.The materials were co-cultured with Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli)respectively for 6 h followed by 808 nm laser irradiation and culture for 6 h,and then the antibacterial rate of the materials was calculated by absorbance detection.Finally,the anti-infection effect was observed 7 days after implantation in the rat femoral defect infection model of SD rats.Results:SC@PDA maintained the silicic acid release characteristics of silicified collagen and had good biocompatibility and good photothermal property.In vitro and in vivo antibacterial experiments showed that SC@PDA had good antibacterial activity without cytotoxicity and acut system toxicity.Conclusion:SC@PDA has biocompatibility and the dopamine coating endows the silicified collagen scaffold with excellent photothermal antibacterial properties.

关 键 词：聚多巴胺 胶原支架 植入物相关感染 抗菌材料 

分 类 号：R783.1[医药卫生—口腔医学]