等离子喷涂钽涂层钛对大鼠骨结合影响的实验研究  

The effects of plasma spraying tantalum-coated titanium on osseointegration in rats

作　　者：吴小燕 于淼[2] 葛潇 毕秀婷 于晨 李倜[2] WU Xiaoyan;YU Miao;GE Xiao;BI Xiuting;YU Chen;LI Ti(School of Stomatology of Weifang Medical University,261000,China;Stomatologogical Hospital of Weifang People's Hospital)

机构地区：[1]潍坊医学院口腔医学院,261000 [2]潍坊市人民医院口腔医院

出　　处：《实用口腔医学杂志》2024年第4期473-479,共7页Journal of Practical Stomatology

基　　金：山东省医药卫生科技发展计划(编号:2019WS250)。

摘　　要：目的:观察纯钛植入体经喷砂酸蚀(SLA)联合等离子喷涂钽涂层处理后在大鼠体内的骨结合及新骨形成情况。方法:纯钛样品抛光清洗后,经喷砂、酸蚀处理,以等离子喷涂技术制备钽涂层。以喷砂酸蚀钛(SLA组)为对照组,钽涂层钛(SLA-Ta组)为实验组。利用扫描电镜、能谱分析仪、接触角测量仪和划痕测试仪对两组材料表面进行表征分析。将两组植入体随机植入大鼠的双侧股骨髁部,术后对大鼠进行双荧光标记。18只大鼠随机分别于术后4、12周处理死(n=9),通过Micro-CT扫描、荧光显微镜观察和组织学染色相结合评价植入体周围新骨形成和骨结合状况。结果:等离子喷涂成功在SLA钛表面制备了微纳米多级结构的钽涂层。体内实验显示,术后4、12周,SLA-Ta组植入体周围新生骨量、骨结合率均明显高于SLA组。结论:SLA联合等离子喷涂钽形成的微纳米多级结构可促进新骨形成,加速其沉积矿化,进而提高植入体的骨结合能力。Objective:To study the osseointegration and new bone formation of titanium implants treated by sandblasting acid etching combined with plasma spraying tantalum coating in rats.Methods:After polishing and cleaning of the pure titanium specimens(SLA),the tantalum coating was prepared by plasma spraying after sandblasting and acid etching(SLA-Ta).The surface feature of the samples was observed by SEM,EDS and contact angle meter.The samples of SLA and SLA-Ta were respectively implanted into femur condyle at each side of 18 SD rats(SLA group and SLA-Ta group),and the rats were double-fluorescent labeled after surgery.The rats were sacrificed 4 and 12 weeks after implantation respectively(n=9),the samples of implants and surrounding tissue were examined by micro-CT scanning,fluorescence microscopy and histological staining,the new bone formation and osseointegration around the implant were analyzed.Results:Micro-nano Porous structureed tantalum coating was successfully prepared on the surface of SLA titanium.In vivo experiments showed that 4 and 12 weeks after implantation,the amount of new bone and osseointegration around the implant in the SLA-Ta group were significantly higher than those in the SLA group.Conclusion:The micro-nano multi-level structure formed by SLA combined with plasma sprayed tantalum can promote new bone formation,accelerate mineralization deposition,and improve the osseointegration capacity of the implant.

关 键 词：钽涂层 骨结合 植入体 表面改性 等离子喷涂 

分 类 号：R783.1[医药卫生—口腔医学]