血小板因子4及肿瘤坏死因子-α在慢性牙周炎发病中的相互影响  

Interaction of platelet factor 4 and tumor necrosis factor-α in the pathogenesis of chronic periodontitis

作　　者：郑欣欣 熊雨 王雪 陈丽珍 陈敬林 钟丽萍 金幼虹[1] ZHENG Xinxin;XIONG Yu;WANG Xue;CHEN Lizhen;CHEN Jinglin;ZHONG Liping;JIN Youhong(Department of Periodontology,The Affiliated Stomatological Hospital of Nanchang University,The Key Laboratory of Oral Biomedicine,Jiangxi Province Clinical Research Center for Oral Diseases,330006,China)

机构地区：[1]南昌大学附属口腔医院牙周科,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,330006

出　　处：《实用口腔医学杂志》2024年第4期557-560,共4页Journal of Practical Stomatology

基　　金：江西省科技厅重点研发计划项目(编号:20192BBG70020);江西省中医药管理局科技计划一般项目(编号:2020B0162);江西省卫健委科技计划课题(编号:SKJP220202269)。

摘　　要：目的:探讨血小板因子4(PF4)和肿瘤坏死因子-α(TNF-α)在慢性牙周炎(Ⅲ期C级)发病中的相互影响。方法:收集慢性牙周炎(Ⅲ期C级)患者22例和牙周健康者22例,ELISA测定牙周治疗前、后龈沟液(GCF)和血清中PF4及TNF-α的浓度和脂多糖(LPS)体外刺激患者治疗前、后外周血的血小板后血小板释放PF4的量;流式细胞仪计算治疗前、后GCF内血小板数量。结果:患者GCF、血清中PF4和TNF-α的浓度高于牙周健康组(P<0.05),治疗后较治疗前GCF中的PF4和TNF-α降低明显(P<0.05)、血清中的PF4和TNF-α无变化(P>0.05)。LPS刺激治疗前、后血液中的血小板后,血小板释放PF4的浓度均远远高于健康对照组(P<0.01),牙周治疗后较治疗前降低明显(P<0.01)。牙周治疗前的GCF中CD41/CD61双阳性血小板数和CD45阴性细胞数分别是牙周健康者的85倍和87倍(P<0.01)。结论:PF4与TNF-α在慢性牙周炎的发病中具有协同效应。Objective:To investigate the interaction of platelet factor 4(PF4)with tumor necrosis factor-α(TNF-α)in the pathoge-nesis of chronic periodontitis(Ⅲ-C).Methods:22 patients with chronic periodontitis(Ⅲ-C)and 22 subjects with periodontal health were recruited.Before and after periodontal treatment,the concentration of PF4 and TNF-αin gingival crevicular fluid(GCF)and serum,the amount of PF4 released by platelets after lipopolysaccharide(LPS)stimulated peripheral blood platelets were measured by ELISA.Flow cytometry was used to calculate the number of platelets in GCF before and after treatment.Results:The concentrations of PF4 and TNF-αin the GCF and serum of the patients were higher than those in the periodontal healthy group(P<0.05).After treatment,the concentrations of PF4 and TNF-αin the GCF were significantly lower than those before treatment(P<0.05),and the concentrations of PF4 and TNF-αin the serum were unchanged(P>0.05).After LPS stimulation of the platelets in blood before and after treatment,the concentration of PF4 released by the platelets was much higher in the patients than that in the healthy controls(P<0.01),and the concentration was significantly lower after periodontal treatment than before treatment(P<0.01).The number of CD41/CD61 double positive platelets and CD45 negative cells in GCF before periodontal treatment were 85 times and 87 times higher than those in periodontal healthy subjects,respectively(P<0.01).Conclusion:PF4 and TNF-αhave synergistic effect in the pathogenesis of chronic periodontitis.

关 键 词：慢性牙周炎(Ⅲ期C级) 牙周基础治疗 血小板因子4 肿瘤坏死因子-Α 血小板聚集 血小板活化 

分 类 号：R781.4[医药卫生—口腔医学]