A 3D In-vitro model of the human dentine interface shows long-range osteoinduction from the dentine surface  

作　　者：William Macalester Asme Boussahel Rafael O.Moreno-Tortolero Mark R.Shannon Nicola West Darryl Hill Adam Perriman 

机构地区：[1]School of Cellular and Molecular Medicine,Biomedical Sciences Building,University of Bristol,University Walk,Bristol,United Kingdom [2]Bristol Centre for Functional Nanomaterials,HH Wills Physics Laboratory,University of Bristol,Tyndall Avenue,Bristol,United Kingdom [3]Centre for Protolife Research,School of Chemistry,University of Bristol,Cantocks Close,Bristol,United Kingdom [4]Max Planck-Bristol Centre for Minimal Biology,School of Chemistry,University of Bristol,Bristol,United Kingdom [5]Periodontology,Bristol Dental School,University of Bristol,Lower Maudlin Street,Bristol,United Kingdom

出　　处：《International Journal of Oral Science》2024年第2期225-237,共13页国际口腔科学杂志（英文版）

基　　金：supported by the Bristol Centre for Functional Nanomaterials and GlaxoSmithKline.

摘　　要：Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral defects.However,the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering,exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems,which will impact on cell fate and subsequent treatment efficacy.Herein,we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells(hMSCs)encapsulated in a microporous hydrogel bioink.We demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs,and that both dentine andβ-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface,which is distal to the gel-substrate interface.Our findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provide a framework for the rapid development of 3D dentine-bone interface models.

关 键 词：INTERFACE DEN SURFACE 

分 类 号：R78[医药卫生—口腔医学]