PRX1-positive mesenchymal stem cells drive molar morphogenesis  

作　　者：Xiaoqiao Xu Xuyan Gong Lei Zhang Han Zhang Yao Sun 

机构地区：[1]Department of Implantology,Stomatological Hospital and Dental School of Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai,China

出　　处：《International Journal of Oral Science》2024年第2期293-305,共13页国际口腔科学杂志（英文版）

基　　金：financial support of the National Natural Science Foundation of China(82270963,82061130222)awarded to Y.S.

摘　　要：Mammalian teeth,developing inseparable from epithelial-mesenchymal interaction,come in many shapes and the key factors governing tooth morphology deserve to be answered.By merging single-cell RNA sequencing analysis with lineage tracing models,we have unearthed a captivating correlation between the contrasting morphology of mouse molars and the specific presence of PRX1^(+)cells within M1.These PRX1^(+)cells assume a profound responsibility in shaping tooth morphology through a remarkable divergence in dental mesenchymal cell proliferation.Deeper into the mechanisms,we have discovered that Wnt5a,bestowed by mesenchymal PRX1^(+)cells,stimulates mesenchymal cell proliferation while orchestrating molar morphogenesis through WNT signaling pathway.The loss of Wnt5a exhibits a defect phenotype similar to that of siPrx1.Exogenous addition of WNT5A can successfully reverse the inhibited cell proliferation and consequent deviant appearance exhibited in Prx1-deficient tooth germs.These findings bestow compelling evidence of PRX1-positive mesenchymal cells to be potential target in regulating tooth morphology.

关 键 词：WNT5A MESENCHYMAL inhibited 

分 类 号：R78[医药卫生—口腔医学]