苏木精-伊红染色切片评估食管鳞状细胞癌中三级淋巴结构的预后意义  

Prognostic significance of tertiary lymphoid structure assessment with hematoxylin-eosin staining in esophageal squamous cell carcinoma

作　　者：梁银凤 王志中 孙瑞 王冬青 赵九洲 王博 苗晋鑫 郭永军 Liang Yinfeng;Wang Zhizhong;Sun Rui;Wang Dongqing;Zhao Jiuzhou;Wang Bo;Miao Jinxin;Guo Yongjun(Department ofMolecular Pathology,Henan Key Laboratory of Molecular Pathology,The Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer Hospital,Zhengzhou 450003,Henan,China)

机构地区：[1]郑州大学附属肿瘤医院&河南省肿瘤医院分子病理科、河南省分子病理学重点实验室,河南郑州450003

出　　处：《肿瘤综合治疗电子杂志》2024年第3期121-127,共7页Journal of Multidisciplinary Cancer Management(Electronic Version)

基　　金：河南省重大公益项目(201300310400);河南省医学科技攻关省部共建青年项目(SBGJ202103031);河南省科技攻关项目(232102311059)。

摘　　要：目的探索利用苏木素-伊红(hematoxylin-eosin,HE)染色切片评估三级淋巴结构(tertiary lymphoid structures,TLS)在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的潜在预后预测价值。方法选取2014—2015年河南省肿瘤医院医院收治的未经过术前放化疗的87例ESCC患者的手术标本,收集临床病理数据。采用HE染色法检测肿瘤区域内TLS的存在情况、密度、最大直径和分布情况。采用Kaplan-Meier生存分析和多因素Cox回归分析评估TLS特征对ESCC患者预后的影响。收集肿瘤基因图谱(Cancer Genome Atlas,TCGA)数据库中84例ESCC患者的临床病理数据对结果进行验证。结果在收集的87例ESCC患者中,58例经检测到在TLS,与TCGA数据库中的84例患者中44例存在TLS的情况一致。TLS的存在及其平均密度、直径和分布与ESCC患者的生存时间延长相关。但在校正病理特征之后,仅TLS的存在状态为ESCC患者中长期生存的独立预后因素(HR=0.432,P=0.004),与TCGA数据库中验证的结果一致(HR=0.359,P=0.028)。结论在ESCC中常规利用HE染色法评估TLS的存在是可行的,且可以作为有效的预后预测生物标志物。Objective To explore the potential prognostic prediction values of tertiary lymphoid structure(TLS)assessed using hematoxylin-eosin staining(HE)sections in esophageal squamous cell carcinoma(ESCC).Method We conducted a comprehensive study involving 87 patients diagnosed with ESCC in Henan Cancer Hospital from 2014 to 2015 without preoperative radiotherapy and chemotherapy.Relevant clinicopathological data were collected.HE staining was performed to investigate the presence,density,diameter,and distribution of TLS within the cancerous regions.Subsequently,survival analysis using Kaplan-Meier and multivariable Cox regression analysis was executed to assess the prognostic significance of TLS profiles in ESCC patients.To corroborate our findings,we validated the results in an additional dataset comprising 84 ESCC patients obtained from the TCGA repository.Result We detected TLS in 58 out of the 87 collected ESCC patients,mirroring the TCGA dataset where 44 out of 84 patients displayed TLS.The presence of TLS,coupled with their average density,diameter and distribution,demonstrated a correlation with prolonged survival in ESCC patients.However,upon adjusting for pathologic features,only the presence status of TLS emerged as an independent prognostic factor for long-term survival in ESCC,yielding a hazard ratio of 0.432(HR=0.432,P=0.004).This finding was validated in the TCGA data set with of hazard ratio of 0.359(HR=0.359,P=0.028).Conclusion The routine evaluation of TLS presence in ESCC with HE staining appears to be feasible and can serve as an efficient biomarker for prognostic prediction.

关 键 词：食管鳞状细胞癌 三级淋巴结构 苏木素-伊红染色法 预后价值 

分 类 号：R735.1[医药卫生—肿瘤]