糯玉米和普通玉米籽粒蛋白差异分析  

Differential Analysis of Gene Expression in Kernels of Glutinous Maize and Common Maize after Pollination

作　　者：陈华峰 刘颖 袁月 张晓 张家宁 刘丹 CHEN Huafeng;LIU Ying;YUAN Yue;ZHANG Xiao;ZHANG Jianing;LIU Dan(Tianjin Key Laboratory of Crop Genetics and Breeding,Tianjin Crop Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China;Tianjin Zhongtiandadi Technology Company Limited,Tianjin 300384,China;Hebei Normal University of Science&Technology,Qinhuangdao,Hebei 066000,China)

机构地区：[1]天津市农业科学院农作物研究所/天津市农作物遗传育种重点实验室,天津300384 [2]天津中天大地科技有限公司,天津300384 [3]河北科技师范学院,河北秦皇岛066000

出　　处：《天津农业科学》2024年第6期7-14,共8页Tianjin Agricultural Sciences

基　　金：天津市青年基金(小麦籽粒醇溶蛋白主效编码基因克隆及功能解析,23JCQNJC00640);2023年天津市农科院种业创新研究(优异鲜食玉米种质资源创制及新品种选育);河北科技师范学院科学研究基金(2024YB020)。

摘　　要：为了探讨玉米糯性是否会影响籽粒储藏蛋白组成及含量,明确糯玉米与普通玉米在基因表达上的差异,为糯玉米育种提供更多的基因资源,通过使用A-PAGE和转录组测序分析等技术,对普通玉米和糯玉米的籽粒储藏蛋白组成及基因表达模式进行了分析。结果表明:糯玉米在籽粒储藏蛋白组成上与普通玉米接近,同时糯玉米和普通玉米的醇溶蛋白迁移速率明显快于小麦籽粒醇溶蛋白。基因表达上,玉米醇溶蛋白Zm00001d005793(mc1)在糯玉米和普通玉米上的表达模式上基本吻合,未见明显差异;玉米醇溶蛋白Zm00001d049243(fl2)和Zm00001d048851(fl4)在糯玉米中表达丰度显著低于普通玉米。以上结果说明,玉米糯性所在的调控通路可能影响籽粒储藏蛋白编码基因的表达。在育种过程中,应关注糯性和籽粒储藏蛋白的变化,从而提升育种产品的多样化,提高市场竞争力。In order to explore whether waxy properties of maize can affect the composition and content of storage protein,and to clarify the difference of gene expression between waxy maize and common maize,so as to provide more gene resources for waxy maize breeding.In this study,A-PAGE and transcriptome sequencing techniques were used to analyze the composition and gene expression patterns of seed storage proteins in common maize and waxy maize..The results showed that the grain storage protein composition of waxy maize was identical to that of common maize,and the gliadin migration rate of waxy maize and common maize was faster than wheat grain gliadin.The expression pattern of Zm00001d005793(mc1)in waxy maize was comparable to that of common maize,although its expression abundance was lower.The expression patterns of zein Zm00001D005793(mc1)in waxy maize and common maize were similar,with no significant difference discovered.The expression of zein Zm00001D049243(FL2)and Zm00001D048851(FL4)was considerably lower in waxy maize than in common maize The results indicated that the regulation pathway of waxy traits in maize may affect the expression of genes encoding grain storage proteins.In breeding,we should pay attention to the changes of waxy properties and seed storage protein,so as to enhance the diversification of breeding products and enhance market competitiveness.

关 键 词：玉米 籽粒储藏蛋白 醇溶蛋白 加工品质 

分 类 号：S326[农业科学—作物遗传育种] S513[农业科学—农艺学]