双菌共培养产氢体系的构建及产氢性能的优化  

Construction and performance of hydrogen production system bythe co-culture of Clostridium butyricum and Enterobacter aerogenes

作　　者：张新 王建米 蒋永琪 付欢 周俊[1,2] 吴夏芫[1] 雍晓雨[1,2] 贾红华[1] ZHANG Xin;WANG Jianmi;JIANG Yongqi;FU Huan;ZHOU Jun;WU Xiayuan;YONG Xiaoyu;JIA Honghua(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;Bioenergy Research Institute,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京工业大学生物与能源研究所,江苏南京211800

出　　处：《生物加工过程》2024年第4期378-387,共10页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划项目(2018YFA0902200);江苏省创新支撑计划国际科技合作/港澳台合作(港澳台科技合作项目,BZ2022052)。

摘　　要：生物发酵产氢是通过高效产氢菌把自然界储存于有机化合物中的化学能转化为氢气的技术。本研究在前期单菌产氢条件优化的基础上,构建丁酸梭菌(Clostridium butyricum LQ25)产气肠杆菌(Enterobacter aerogenes AJ110637)共培养发酵产氢体系。以葡萄糖为底物,研究共培养发酵体系的产氢性能及其协同效应。结果表明:C.butyricum LQ25与E.aerogenes AJ110637在接菌的体积比9∶1、初始pH 7.0、发酵温度40℃的条件下产氢效率最高,每克葡萄糖产氢量最高可以达到354.05 mL,分别比C.butyricum和E.aerogenes单独发酵高出31.71%和70.59%。在共培养发酵体系中添加1.0 g/L的CaCO 3,可以显著缓解体系的酸化程度,H 2产率比对照组提高了21.80%。与单一菌株纯培养发酵产氢相比,双菌共培养体系产氢率有所提高,体现了C.butyricum和E.aerogenes的协作效应。Bio-hydrogen production is a technology to convert the chemical energy stored in organics to hydrogen by the efficient hydrogen producing bacteria in nature.Based on the previous studies on the optimization of hydrogen production conditions for single hydrogen producing bacteria,a co-culture system for hydrogen production was constructed using C lostridium butyricum LQ25 and Enterobacter aerogenes AJ110637,and the corresponding hydrogen production performance and synergistic effect were subsequently evaluated with glucose as the substrate.As a result,the co-culture system of C.butyricum LQ25 and E.aerogenes AJ110637 could provide the highest hydrogen production efficiency under the conditions with the inoculum ratio of 9∶1,initial pH 7.0,and fermentation temperature of 40℃,with a maximum hydrogen production of 354.05 mL H 2/g glucose,which was 31.71%and 70.59%higher than C.butyricum and E.aerogenes systems,respectively.Notably,the addition of 1.0 g/L calcium carbonate could significantly increase the hydrogen yield by 21.80%compared with the control group,likely through alleviating the acidity of the co-culture system.Overall,the hydrogen production rate in the co-culture system of C.butyricum and E.aerogenes was higher than either single system,reflecting the synergistic effect of C.butyricum and E.aerogenes to a certain extent.

关 键 词：丁酸梭菌 产气肠杆菌 产氢 发酵 共培养 

分 类 号：Q815[生物学—生物工程] Q89