乳酸肠球菌冻干保护剂的优化研究及菌粉保存效果的研究  

Optimization of Freeze-drying Protective Agents for Enterococcus lactis and Preservation Effect of Bacterial Powder

作　　者：张菊 李珍祖[2] 陈焱 郭洪梅 范振先 苏成文 ZHANG Ju;LI Zhenzu;CHEN Yan;GUO Hongmei;FAN Zhenxian;SU Chengwen(Shandong Vocational College of Animal Science and Veterinary Medicine,Weifang,Shandong 261061,China;School of Bioengineering,Shandong Polytechnic,Ji'nan,Shangdong 250014,China)

机构地区：[1]山东畜牧兽医职业学院,山东潍坊261061 [2]山东职业学院生物工程学院,山东济南250014

出　　处：《家畜生态学报》2024年第8期47-52,共6页Journal of Domestic Animal Ecology

基　　金：山东省生猪产业技术体系(SDAIT-08-08)。

摘　　要：为了提高乳酸肠球菌在真空冷冻干燥过程中的存活率以及后期菌粉保存的效果,采用单因素试验和Box-Behnken响应面试验相结合的方法,筛选和优化乳酸肠球菌真空冷冻干燥过程中的最优保护剂配方。结果表明:脱脂奶粉、海藻糖和甘油3种保护剂效果较好,能够显著提高乳酸肠球菌真空冷冻干燥后的存活率。将上述3种保护剂作为自变量,以冻干后菌体的存活率作为响应值进行响应面试验,得到乳酸肠球菌优化保护剂配方:脱脂奶粉20.82%、海藻糖13%、甘油3%,通过进一步验证和保存试验研究,采用优化后的保护剂配方,菌体冻干后的存活率均在80.00%左右,冻干菌粉在4℃条件下可以保存24个月。In order to improve the survival rate of Enterococcus lactate and the preservation effect of bacterial powder in later period during vacuum freeze-drying,the single factor experiment and Box-Behnken design was used to screen and optimize the protective agents formula for vacuum freeze-drying of Enterococcus lactate.The results showed that the skim milk,trehalose and glycerin have the best effect,which can significantly improve the survival of Enterococcus lactate after vacuum freeze-drying.In response surface experiment of Box-Behnken,the above three protective agents were taken as independent variables,and the survival rate of cells after lyophilization was the response value.The optimized protective agent formula of Enterococcus lactate was obtained:skim milk 20.82%,trehalose 13%,glycerin 3%.Further verification and stability research showed that the survival rate of lyophilized thallus reached about 80.00%with the optimized protective agent formula,the lyophilized Enterococcus lactate powder can be preserved for 24 months at 4℃.

关 键 词：乳酸肠球菌 真空冷冻干燥 保护剂 BOX-BEHNKEN设计 

分 类 号：S816.73[农业科学—饲料科学]