林下种植蜜花豆生长早期的类黄酮化合物含量特征  

Content Characteristics of Flavonoids in Spatholobus suberectus Planted Under Forest at Early Growth Stage

作　　者：周启华 曹秋婵 黄春晖 杨开太[3] 刘军 白天道[2] ZHOU Qihua;CAO Qiuchan;HUANG Chunhui;YANG Kaitai;LIU Jun;BAI Tiandao(Guangxi State-owned Qipo Forest Farm,Nanning,Guangxi 530225;Guangxi Key Laboratory of Forest Ecology and Conservation,College of Forestry of Guangxi University,Nanning,Guangxi 530004;Guangxi Academy of Forestry,Nanning,Guangxi 530009,China)

机构地区：[1]广西壮族自治区国有七坡林场,广西南宁530225 [2]广西森林生态与保育重点实验室,广西大学林学院,广西南宁530004 [3]广西林业科学研究院,广西南宁530009

出　　处：《贵州农业科学》2024年第7期90-99,共10页Guizhou Agricultural Sciences

基　　金：广西重点研发计划项目“广西低丘林区林下经济有机高效栽培模式研究与示范”(桂科AB18221003);“广西林下经济特色种质资源收集保存与生态高效栽培及利用研究”(桂科AB21238014)。

摘　　要：【目的】探明林下人工栽培蜜花豆(鸡血藤)生长表现和主要药用成分含量的相关性,为林下蜜花豆种植管理及其采收提供参考依据。【方法】采用高效液相色谱法(HPLC法)测定3年生蜜花豆藤茎不同径级、分枝等级和保存方式下5种类黄酮化合物(原儿茶酸、儿茶素、表儿茶素、甘草素和芒柄花素)含量,结合方差分析、主成分分析及曲线回归等方法分析类黄酮化合物在不同处理水平间的差异及变化趋势。【结果】除表儿茶素外,其余4种化合物在蜜花豆不同径级藤茎间均存在显著差异,随藤径级增大,原儿茶酸、儿茶素和芒柄花素含量呈线性增加,甘草素含量呈非线性下降;原儿茶酸、甘草素和芒柄花素含量在蜜花豆不同分枝等级间存在显著差异,随分枝等级增加,原儿茶酸、儿茶素、芒柄花素及表儿茶素含量降低,甘草素含量则增加;蜜花豆藤茎风干样中儿茶素、表儿茶素含量显著高于冷藏鲜样。【结论】原儿茶酸、儿茶素和芒柄花素主要在木质部富集,甘草素则主要存在于非木质部组织,蜜花豆风干保存有利于富集儿茶素和表儿茶素。【Objective】The relationship between the growth performance and the content of the main medicinal components in the artificial cultivation of Spatholobus suberectus planted under the forest were studied,which provided a theoretical reference for planting management and harvesting S.suberectus planted under the forest.【Method】The contents of five flavonoid compounds(protocatechuic acid,catechin,epicatechin,liquiritigenin and formononetin)in 3-yearold S.suberectus with different diameter,branching classes and rattan preservation methods were detected by high performance liquid chromatography(HPLC)method.Analysis of variance(ANOVA),principal component analysis(PCA)and curvilinear regression analysis were used to reveal the difference and variation trend of the five flavonoids contents under different treatments.【Result】There were significant differences in four flavonoids among diameter classes of rattan of S.suberectus except epicatechin.The contents of protocatechuic acid,catechin and formononetin increased linearly as the diameter class of rattan increased,while the liquiritigenin non-linearly decreased with the diameter of rattan;The contents of protocatechuic acid,liquiritigenin and formononetin significantly differed among different branch grades of S.suberectus.The protocatechuic acid,catechin,formononetin and epicatechin decreased with the increase of branch grades,while the content of liquiritigenin showed a reverse tendency.The contents of catechin and epicatechin in air-dried samples of S.suberectus were significantly higher than those in refrigerated fresh samples.【Conclusion】The protocatechin acid,catechin and formononetin mainly exist in xylem.On the contrary,the liquiritigenin primarily locates at non-xylem area.The air-drying preservation of S.suberectus is beneficial to the accumulation of catechin and epicatechin.

关 键 词：蜜花豆 藤径 药用植物 分枝等级 类黄酮 HPLC 林下种植 

分 类 号：S567.19[农业科学—中草药栽培]