Glycnsisitin A:A promising bicyclic peptide against heart failure that facilitates TFRCmediated uptake of iron in cardiomyocytes  

作　　者：Jichao Zhou Yuanyuan Liu Xiaoli Wei Meng Yuan Xu Zhang Lingfeng Qin Bing Cui Pingping Li Jing Zhang Ziming Feng Jianshuang Jiang Xiang Yuan Ruibing Xu Zhimeng Zhang Peicheng Zhang Xiaowei Zhang Yanan Yang 

机构地区：[1]State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China

出　　处：《Acta Pharmaceutica Sinica B》2024年第7期3125-3139,共15页药学学报（英文版）

基　　金：supported by Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2022-I2M-2-002,2021-I2M-1-016,and 2022-I2M-1-014,China);the Beijing Outstanding Young Scientist Program(BJJWZYJH01201910023028,China);Chinese Academy of Medical Sciences(CAMS)Central Public-interest Scientific Institution Basal Research Fund(2018PT35004,Molecular Mechanism and Target Discovery of Metabolic Disorder and Tumorigenesis,CAMS Key Laboratory).

摘　　要：Zhigancao decoction is a traditional prescription for treating irregular pulse and palpitations in China.As the monarch drug of Zhigancao decoction,the bioactive molecules of licorice against heart diseases remain elusive.We established the HRESIMS-guided method leading to the isolation of three novel bicyclic peptides,glycnsisitins A-C(1-3),with distinctive C-C and C-O-C side-chain-toside-chain linkages from the roots of Glycyrrhiza uralensis(Licorice).Glycnsisitin A demonstrated stronger cardioprotective activity than glycnsisitins B and C in an in vitro model of doxorubicin(DOX)-induced cardiomyocyte injury.Glycnsisitin A treatment not only reduced the mortality of heart failure(HF)mice in a dose-dependent manner but also significantly attenuated DOX-induced cardiac dysfunction and myocardial fibrosis.Gene set enrichment analysis(GSEA)of the differentially expressed genes indicated that the cardioprotective effect of glycnsisitin A was mainly attributed to its ability to maintain iron homeostasis in the myocardium.Mechanistically,glycnsisitin A interacted with transferrin and facilitated its binding to the transferrin receptor(TFRC),which caused increased uptake of iron in cardiomyocytes.These findings highlight the key role of bicyclic peptides as bioactive molecules of Zhigancao decoction for the treatment of HF,and glycnsisitin A constitutes a promising therapeutic agent for the treatment of HF.

关 键 词：Glycyrrhiza uralensis Bicyclic peptides Myocardial energy metabolism Iron homeostasis TFRC 

分 类 号：R542.2[医药卫生—心血管疾病] R96[医药卫生—内科学]