大黄多糖的提取及其抗氧化和降血糖活性研究  

Extraction of Rhubarb Polysaccharide and their Antioxidant and Hypog lycemic Activities

作　　者：王芳 刘红 李燕红 龚田 WANG Fang;LIU Hong;LI Yan-hong;GONG Tian(Gansu Medical University,Pingliang 744000,China)

机构地区：[1]甘肃医学院,甘肃平凉744000

出　　处：《海峡药学》2024年第7期8-12,共5页Strait Pharmaceutical Journal

基　　金：甘肃省高等学校创新基金项目(NO.2022A-184);甘肃省高等学校创新基金项目(NO.2023B-263)。

摘　　要：目的从中药大黄中提取大黄多糖(Rhubarb polysaccharide,RPS),并研究其体外对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)与1,1-二苯基-2-苦基肼(DPPH)的自由基清除能力,以及对α-葡萄糖苷酶和胰α-淀粉酶的抑制作用,进而评估RPS在治疗抗氧化及糖尿病等方面的潜在价值。方法通过水提醇沉法提取RPS,用苯酚-硫酸比色法测定多糖含量,并通过分光光度法和酶联免疫检测法评定其抗氧化能力及降血糖活性。结果大黄中多糖的平均含量值为5.01%±1.44%、蛋白质含量约为7.17%,平均回收率为96.41%±1.08%。体外抗氧化实验显示,RPS具有很好的AB TS与DPPH自由基清除活性,且优于维生素C(Vc)。降血糖实验结果表明,当RPS浓度在0.25 mg·mL^(-1)、0.50 mg·mL^(-1)、1.00 mg·mL^(-1)时,在以4-硝基-α-D-呋喃葡萄糖苷溶液(p-Nitrophenyl-α-D-Galactopyranoside,PNPG)为底物的α-葡萄糖苷酶-抑制剂模型上,和以可溶性淀粉为底物的胰α-淀粉酶-抑制剂模型上,RPS的抑制率分别为25.6%,31.2%,38.0%和28.62%,34.50%,50.73%,且有一定的量效关系。结论RPS具有良好的抗氧化和降血糖活性,这为其在医药和食品领域的应用提供了有力支持。OBJECTIVE This study aims to extract Rhubarb polysaccharide(RPS)from Chinese medicine Rhubarb and investigate its scavenging abilities against 2,2-diazo-di(3-ethylbenzothiazole-6-sulfonic acid)diamine salt(ABTS)and 1,1-diphenyl-2-picrylhydrazine(DPPH)radicals in vitro,as well as its inhibitory effects onα-glu-cosidase and pancreaticα-amylase,thereby evaluating the potential value of RPS in the treatment of antioxidation and diabetes.METHODS RPS was extracted by water extraction and alcohol precipitation.The polysaccharide content was determined using the phenol-sulfuric acid colorimetry,and its antioxidant capacity and hypoglycemic activity were measured spectrophotometrically.RESULTS The average content of polysaccharide in Rhubarb was 5.01%±1.44%,the protein content was approximately 7.17%,and the average recovery rate was 96.41%±1.08%.Antiox-idant experiments in vitro showed that RPS exhibited good scavenging activities against ABTS and DPPH radicals,su-perior to vitamin C(Vc).The results of hypoglycemic experiments in vitro showed that when the RPS concentration was 0.25 mg·mL^(-1),0.50 mg·mL^(-1)and 1.00 mg·mL^(-1),the inhibitory rates of RPS onα-glucosidase and pan-creaticα-amylase were 25.6%,31.2%,38.0%and 28.62%,34.50%,50.73%respectively,in theα-glucosidase inhibitor model using p-nitrophenyl-α-D-galactopyranoside(PNPG)as the substrate and the pancreaticα-amylase inhibitor model using soluble starch as the substrate.CONCLUSION RPS exhibits good antioxidant and hypoglyce-mic activities,providing strong support for its application in the fields of medicine and food.

关 键 词：大黄多糖(RPS) Α-糖苷酶 胰α-淀粉酶 ABTS自由基 DPPH自由基 

分 类 号：R965[医药卫生—药理学]