下咽鳞状细胞癌组织miR-204、miR-504表达与临床病理特征和预后的关系  

Relationship Among the Expressions of miR-204 and miR-504 in Hypopharyngeal Squamous Cell Carcinoma Tissue and Clini-copathological Features and Prognosis

作　　者：秦隆朝 赵倩 任姣姣 要兆旭[1] 韩海平[1] QIN Longchao;ZHAO Qian;REN Jiaojiao;YAO Zhaoru;HAN Haiping(Second Department of Otolaryngology Head and Neck Surgery,Handan Central Hospital,Handan Hebei 056000,China)

机构地区：[1]邯郸市中心医院耳鼻咽喉头颈外科二科,河北邯郸056000

出　　处：《联勤军事医学》2024年第5期380-384,407,共6页Military Medicine of Joint Logistics

基　　金：河北省2024年度医学科学研究课题计划(20240881)。

摘　　要：目的 探讨下咽鳞状细胞癌(hypopharyngeal squamous cell carcinoma, HSCC)组织微小核糖核酸204(microRNA-204,miR-204)、miR-504表达与临床病理特征和预后的关系。方法 选取2018-04/2020-04月作者医院收治的122例HSCC患者为研究对象。收集所有患者术中癌组织及癌旁组织标本(距肿瘤切缘≥2 cm),采用逆转录聚合酶链反应(reverse transcription polymerase chain reaction, RT-PCR)检测癌组织及癌旁组织中miR-204、miR-504相对表达量。分析miR-204、miR-504表达与HSCC患者临床病理特征的关系。对HSCC患者进行3年随访。采用单因素和多因素Cox比例风险回归模型分析HSCC患者预后的影响因素。绘制Kaplan-Meier生存曲线,分析miR-204、miR-504低表达和高表达HSCC患者3年总生存情况。结果 与癌旁组织比较,HSCC癌组织miR-204、miR-504表达显著降低(P均<0.05)。不同性别、年龄、吸烟史、饮酒史、T分期、发病部位、淋巴结个数、结外侵犯、术后同步放化疗的HSCC患者miR-204、miR-504表达差异无统计学意义(P>0.05),N1~N3期、低分化程度HSCC患者的miR-204、miR-504表达显著低于N0期、中高分化程度HSCC患者(P均<0.05)。Cox回归分析结果显示,T分期和N分期升高、阳性淋巴结个数>2个、伴结外侵犯、低分化程度、miR-204表达降低、miR-504表达降低是HSCC患者预后不良的危险因素(P<0.05)。122例HSCC患者随访3年期间失访5例,最终获得随访117例。Kplan-Meier生存曲线分析显示,miR-204、miR-504低表达组3年总生存率低于高表达组[54.29%(38/70)vs. 70.21%(33/47),P<0.05;50.68%(37/73)vs. 77.27%(34/44,P<0.05]。结论 HSCC组织中miR-204、miR-504均呈低表达,二者低表达与HSCC患者的T分期和N分期升高、低分化程度及预后不良有关。Objective To investigate the relationship among the expressions of microRNA-204(miR-204),miR-504 in hypopharyngeal squamous cell carcinoma(HSCC)tissue and clinicopathological features and prognosis.Methods A total of 122 HSCC patients who were admitted to author's hospital from April 2018 to April 2020 were selected.The intraoperative cancer tissues and cancer adjacent tissues(≥2 cm from the tumor resection margin)were collected,the rel-ative expression levels of miR-204 and miR-504 in cancer tissues and cancer adjacent tissues were detected by reverse tran-scription polymerase chain reaction(RT-PCR).The relationship between the expressions of miR-204,miR-504 and the clinicopathological features of HSCC patients was analyzed.HSCC patients were followed up for 3 years.The prognostic factors of HSCC patients were analyzed by univariate and multivariate Cox proportional hazard regression models.Kaplan-Meier survival curve was drawn,3 years overall survival of HSCC patients with low expression and high expression of miR-204 and miR-504 were analyzed.Results Compared with adjacent tissues,the expression of miR-204 and miR-504 in HSCC cancer tissue significantly decreased(all P<0.05).There was no statistically significant difference in the expres-sions of miR-204 and miR-504 among HSCC patients with different gender,age,smoking history,alcohol consumption history,T stage,site of onset,the number of lymph nodes,extranodal extension and postoperative chemoradiation(P>0.05),the expressions of miR-204 and miR-504 in N1-N3 stage and poorly differentiated HSCC patients were significant-ly lower than that in N0 stage and medium to high differentiated HSCC patients(all P<0.05).The Cox regression analy-sis results showed that elevated T and N stages,positive lymph nodes>2,extranodal extension,low differentiation,decreased miR-204 expression and de-creased miR-504 expression were risk factors for poor prognosis in HSCC patients(P<0.05).During a 3-year follow-up period,5 out of 122 HSCC patients were lost and 117 were ultimatel

关 键 词：下咽鳞状细胞癌 微小核糖核酸204 微小核糖核酸504 病理特征 预后 

分 类 号：R739.63[医药卫生—肿瘤] R762[医药卫生—临床医学]