氧化石墨烯对芸豆花期碳氮代谢及生长的影响  

Effect of graphene oxide on carbon and nitrogen metabolism and growth of kidney bean at flowering stage

作　　者：于洋 孙旭冉 王诗雅 李沐恺 孙海燕[1,2] 郭伟 YU Yang;SUN Xuran;WANG Shiya;LI Mukai;SUN Haiyan;GUO Wei(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China;Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement,Daqing 163319,Heilongjiang,China;Key Laboratory of Low-carbon Green Agriculture in Northeastern China,Ministry of Agriculture and Rural Affairs,Daqing 163319,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]黑龙江省现代农业栽培技术与作物种质改良重点实验室,黑龙江大庆163319 [3]农业农村部东北平原农业绿色低碳重点实验室,黑龙江大庆163319

出　　处：《应用生态学报》2024年第7期1843-1849,共7页Chinese Journal of Applied Ecology

基　　金：黑龙江八一农垦大学“揭榜挂帅”科技攻关项目(JB20220001);黑龙江省大学生创新训练计划项目(S202310223119)资助。

摘　　要：氧化石墨烯(GO)作为一种新型纳米材料已经应用于不同领域,但作为叶面肥在农业领域的应用较少。本研究通过盆栽试验,分析了始花期叶面喷施0(CK)、50(T_(1))、100(T_(2))、150(T_(3))和200 mg·L^(-1)(T_(4))GO对芸豆植株形态建成和碳氮代谢的影响,以明确叶面喷施GO的生理效应。结果表明:T_(1)~T_(4)处理可不同程度地提高芸豆干物质积累量、光合色素含量和可溶性糖含量,较CK分别显著提高40.7%~43.4%、10.4%~80.7%和6.4%~9.1%,且T_(3)处理影响效果最佳。与CK相比,T_(3)和T_(4)处理下蔗糖磷酸合成酶、酸性转化酶和中性转化酶活性分别显著增加25.7%~45.5%、17.4%~28.6%和14.7%~20.1%,T_(2)和T_(3)处理下硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶活性分别显著增加8.1%~15.2%、11.5%~25.0%和89.7%~93.1%。综上,芸豆始花期叶面喷施适宜浓度的GO可增加光合色素含量,提高植株光合碳、氮代谢水平,进而促进干物质积累,本研究中T_(3)(150 mg·L^(-1))处理的作用效果最好。Graphene oxide(GO)is a novel nanomaterial being applied in different fields,but was less used as foliar fertilizer in agriculture.We conducted a pot experiment to analyze the effects of foliar spraying GO from 0(control),50(T_(1)),100(T_(2)),150(T_(3))and 200 mg·L^(-1)(T_(4))on the morphogenesis and carbon and nitrogen metabolism of kidney bean plants during the initiation of flowering to clarify the physiological effects of foliar spraying GO.The results showed that dry matter accumulation,the content of photosynthetic pigments,soluble sugars of T_(1) to T_(4) treatments,were significantly increased by 40.7%-43.4%,10.4%-80.7%,6.4%-9.1%in kidney bean plants compared with CK treatment,respectively.T_(3) treatment performed the best.Meanwhile,the activities of sucrose phosphate synthase,acid converting enzyme and neutral converting enzyme of T_(3) and T_(4) treatments were increased by 25.7%-45.5%,17.4%-28.6%,and 14.7%-20.1%,and the activities of nitrate reductase,glutamine synthetase,and glutamate synthetase of T_(2) and T_(3) treatments were increased by 8.1%-15.2%,11.5%-25.0%,and 89.7%-93.1%,respectively.In conclusion,foliar spraying of appropriate GO in early flowering stage of kidney bean could increase the content of photosynthetic pigments,improve the level of photosynthetic carbon and nitrogen metabolism,and increase dry matter accumulation.T_(3) treatment(150 mg·L^(-1))was the most effective in this study.

关 键 词：芸豆 氧化石墨烯 碳代谢 氮代谢 

分 类 号：S643.1[农业科学—蔬菜学]