基于Wnt/β-catenin信号通路探讨MEBT/MEBO促进慢性难愈合创面愈合的机制  

MEBT/MEBO promotes the healing of chronic refractory wounds via Wnt/β-catenin signaling pathway

作　　者：左远娟 黄金梅 丘平 黄文华 龚元勋 包崇婵 唐强[3] 唐乾利[1,3] ZUO Yuanjuan;HUANG Jinmei;QIU Ping;HUANG Wenhua;GONG Yuanxun;BAO Chongchan;TANG Qiang;TANG Qianli(Graduate School of Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;Fangchenggang Hospital of Traditional Chinese Medicine,Fangchenggang 538021,Guangxi,China;Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530200 [2]防城港市中医医院,广西防城港538021 [3]右江民族医学院附属医院,广西百色533000

出　　处：《现代中西医结合杂志》2024年第12期1622-1628,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(82160907);广西特聘专家项目(桂人才通字[2019]13号);广西医学高层次领军人才培养“139”计划项目(桂卫科教发[2018]22号);广西中医药大学研究生教育创新计划项目(桂学位[2019]19号)(xjzd025)。

摘　　要：目的 探讨皮肤再生医疗技术/湿润烧伤膏(MEBT/MEBO)影响Wnt/β-catenin信号通路中糖原合酶激酶-3β(GSK-3β)、Wnt3a表达以促进慢性难愈合创面修复的机制。方法将60只Wistar雄性大鼠随机分为空白组、急性创面组、慢性难愈合创面组、MEBT/MEBO组、贝复济组,每组12只。空白组大鼠不进行损伤性处理;其余组大鼠均在统一部位进行全层皮肤切除建立创面,之后慢性难愈合创面组、MEBT/MEBO组、贝复济组大鼠注射醋酸氢化可的松建立慢性难愈合创面。然后每天进行2次清创换药,MEBT/MEBO组、贝复新组创面分别外敷2层创面大小的MEBO纱条、贝复新浸透纱布,其余组外敷2层创面大小的生理盐水纱布,最后覆盖2层消毒的干纱布。统计各组大鼠换药第3,7,14天的创面愈合率,分别采用RT-PCR和Western blot法检测各组大鼠换药第3,7,14天创面组织中GSK-3β、Wnt3a mRNA和蛋白表达情况,免疫组化染色观察慢性难愈合创面组和MEBT/MEBO组换药第14天创面组织中GSK-3β、Wnt3a阳性表达情况。结果 急性创面组、MEBT/MEBO组、贝复新组各时间点的创面愈合率均明显高于慢性难愈合创面组(P均<0.05);MEBT/MEBO组、贝复新组换药第7天的创面愈合率和MEBT/MEBO组换药第14天的创面愈合率均明显高于急性创面组(P均<0.05)。换药第3天,MEBT/MEBO组和贝复新组创面组织中GSK-3β mRNA和蛋白相对表达量均明显低于慢性难愈合创面组(P均<0.05);换药第7天,MEBT/MEBO组、贝复新组GSK-3β mRNA和蛋白相对表达量与慢性难愈合创面组比较差异均无统计学意义(P均>0.05);换药第14天,MEBT/MEBO组和贝复新组GSK-3β mRNA和蛋白相对表达量均明显高于慢性难愈合创面组(P均<0.05)。换药第3,7天,MEBT/MEBO组、贝复新组Wnt3a mRNA和蛋白相对表达量均明显高于慢性难愈合创面组(P均<0.05);换药第14天,MEBT/MEBO组、贝复新组Wnt3a mRNA和蛋白相对表达量均明显低于慢性难愈合创�Objective It is to investigate the mechanism of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)in promoting the healing of chronic refractory wounds via influencing the expression of glycogen synthase kinase-3β(GSK-3β)and Wnt3a in the Wnt/β-catenin signaling pathway.Methods Sixty Wistar male rats were randomly divided into blank group,acute wound group,chronic refractory wound group,MEBT/MEBO group,and rb-bFGF group,with 12 rats in each group.The rats in the blank group were not subjected to any injurious treatment;the rats of the rest group were subjected to total skin excision at a uniform site to create wounds,in which the rats in the acute wound group were not injected with hydrocortisone,and the rats in the other groups were injected with hydrocortisone acetate to create chronic refractory wounds.Then,the wound was cleaned and changed,twice per day,two layers of wound-sized MEBO gauze and rb-bFGF-impregnated gauze were applied to the wound in the MEBT/MEBO group and rb-bFGF group respectively,two layers of wound-sized saline gauze were applied to the wound in the rest groups,and two layers of sterilized dry gauze were finally applied on the surface.The healing rate of wounds on the 3rd,7th and 14th day of drug exchange was calculated in each group,and the mRNA and protein expressions of GSK-3βand Wnt3a in the wound tissue were respectively detected by RT-PCR and Western blot on the 3rd,7th and 14th day of drug exchange in each group,and the positive expressions of GSK-3βand Wnt3a in the wound tissue were detected by immunohistochemical staining on the 14th day of drug exchange in the chronic refractory wound group and MEBT/MEBO group.Results The wound healing rates at each time point in the acute woud group,MEBT/MEBO group and rb-bFGF group were significantly higher than those in the chronic refractory wound group(all P<0.05);the wound healing rates at the 7th day of dressing change in the MEBT/MEBO group and rb-bFGF group,and the wound healing rate at the 14th day of dressing change i

关 键 词：慢性难愈合创面 皮肤再生医疗技术 湿润烧伤膏 糖原合酶激酶-3Β WNT3A 

分 类 号：R-332[医药卫生]