基于UBA2/PTEN/PI3K/Akt通路探讨蔓荆子黄素对结直肠癌细胞增殖、迁移和侵袭的影响  

Effects of vitexicarpin on proliferation, migration and invasion of colorectal cancer cells via UBA2/PTEN/PI3K/Akt pathway

作　　者：张东姣 曹伟 田志刚 樊丽伟 张磊[1] 汪景坤 王静[1] ZHANG Dongjiao;CAO Wei;TIAN Zhigang;FAN Liwei;ZHANG Lei;WANG Jingkun;WANG Jing(The Hospital of 82nd Army Group of PLA,Baoding 071000,Hebei,China;Baoding No2.Central Hospital,Zhuozhou 072750,Hebei,China)

机构地区：[1]中国人民解放军陆军第八十二集团军医院,河北保定071000 [2]保定市第二中心医院,河北涿州072750

出　　处：《现代中西医结合杂志》2024年第12期1629-1634,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：2021年保定市科技计划项目(2141ZF337)。

摘　　要：目的 基于泛素样修饰激活酶2(UBA2)/磷酸酶及张力蛋白同源物(PTEN)/磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)通路探究蔓荆子黄素对结直肠癌SW480细胞增殖、迁移和侵袭的影响。方法 取对数生长期的SW480细胞,对照组细胞常规培养,蔓荆子黄素组细胞加入10μmol/L蔓荆子黄素培养,UBA2抑制剂组细胞加入0.5μmol/L UBA2抑制剂培养,蔓荆子黄素+UBA2抑制剂组细胞加入10μmol/L蔓荆子黄素和0.5μmol/L UBA2抑制剂共培养。CCK-8实验检测细胞增殖情况,克隆形成实验观察细胞的单克隆形成能力,划痕实验观察细胞的迁移能力,Transwell实验观察细胞的侵袭能力,Western blot法检测细胞中UBA2/PTEN/PI3K/Akt通路相关蛋白表达情况。结果 CCK-8实验和克隆形成实验显示,UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组培养72 h后的细胞增殖吸光度OD值明显低于蔓荆子黄素组(P均<0.05),细胞克隆形成数量均明显少于蔓荆子黄素组(P均<0.05),UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组培养不同时间的细胞增殖吸光度OD值和细胞克隆形成数量比较差异均无统计学意义(P均>0.05)。划痕实验和Transwell实验显示,UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组划痕间距均明显宽于蔓荆子黄素组(P均<0.05),穿膜细胞数量均明显少于蔓荆子黄素组(P均<0.05),UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组比较差异均无统计学意义(P均>0.05)。蔓荆子黄素组、UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组细胞中PTEN蛋白相对表达量均明显高于对照组(P均<0.05),UBA2、p-PI3K、p-Akt蛋白相对表达量均明显低于对照组(P均<0.05);UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组细胞中PTEN蛋白相对表达量均明显高于蔓荆子黄素组(P均<0.05),UBA2、p-PI3K、p-Akt蛋白相对表达量均明显低于蔓荆子黄素组(P均<0.05),UBA2抑制剂组和蔓荆子黄素+UBA2抑制剂组UBA2、PTEN、p-PI3K、p-Akt蛋白相对表达量比较差�Objective It is to explore the effects of vitexicarpin on the proliferation,migration and invasion of colorectal cancer SW480 cells based on the ubiquitin-like modifier activating enzyme 2(UBA2)/phosphate and tension homology deleted on chromosome ten(PTEN)/phosphate idylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway.Methods SW480 cells in logarithmic growth phase were taken and randomly divided into 4 groups.The cells in the control group were routinely cultured,the cells in the vitexicarpin group were cultured with 10μmol/L vitexicarpin,the cells in the UBA2 inhibitor group were cultured with 0.5μmol/L UBA2 inhibitor,and the cells in the vitexicarpin+UBA2 inhibitor group were co-cultured with 10μmol/L vitexicarpin and 0.5μmol/L UBA2 inhibitor.The cell proliferation was detected by CCK-8 assay,the monoclonal formation ability of the cells was observed by clone formation assay,the migration ability of the cells was observed by scratch assay,the invasion ability of the cells was observed by Transwell assay,and the expressions of proteins related to UBA2/PTEN/PI3K/Akt pathway in the cells were detected by Western blot.Results CCK-8 assay and clone formation assay showed that the cell proliferation absorbance OD values of the UBA2 inhibitor group and the vitexicarpin+UBA2 inhibitor group were significantly lower,while the numbers of cell clone formation were significantly less than those of the vitexicarpin group(all P<0.05),and the differences in the cell proliferation absorbance OD values and number of cell clone formation were not statistically significant between the UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group at different times of culture(all P>0.05).Scratch and Transwell experiments showed that the scratch spacings in the UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group were significantly wider,while the numbers of membrane-penetrating cells were significantly less than those in the vitexicarpin group(both P<0.05),and the differences were not statistically significant between

关 键 词：蔓荆子黄素 SW480细胞 泛素样修饰激活酶2 磷酸酶及张力蛋白同源物 磷脂酰肌醇3-激酶 蛋白激酶B 

分 类 号：R-33[医药卫生]