激活蛋白激酶B/鼠双微体基因2/p53信号通路抑制非小细胞肺癌H1975细胞增殖和侵袭  

Activation of protein kinase B/murine double minute 2/p53 signaling pathway inhibits proliferation and invasion of non-small cell lung cancer H1975 cells

作　　者：潘宇 傅静云 陈涛[1] Pan Yu;Fu Jingyun;Chen Tao(School of Basic Medicine,Three Gorges University,Yichang 443000,China)

机构地区：[1]三峡大学基础医学院,宜昌443000

出　　处：《中华实验外科杂志》2024年第6期1237-1240,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81873148)。

摘　　要：目的观察调控蛋白激酶B(Akt)/鼠双微体基因2(MDM2)/p53信号通路对非小细胞肺癌(NSCLC)恶性生物学行为的影响。方法取对数期H1975细胞株(美国类型培养物保藏中心),分别用Akt/MDM2/p53信号通路激活剂SC79(4μg/ml)、抑制剂perifosine(5.0μmol/L)处理,设为SC79组、perifosine组,取不做任何处理的H1975细胞株设为空白组。干预24 h检测细胞增殖能力、凋亡率及侵袭能力。多组间比较用单因素方差分析,两组间比较用LSD-t检验。结果SC79组24、48、72 h吸光度值(0.38±0.05、0.63±0.07、0.89±0.12)高于空白组(0.25±0.04、0.46±0.07、0.62±0.11,t=4.540、3.840、3.709,P<0.01);perifosine组24、48、72 h吸光度值(0.16±0.04、0.29±0.06、0.40±0.09)低于空白组(0.25±0.04、0.46±0.07、0.62±0.11,t=3.558、4.123、3.461,P<0.01)。SC79组凋亡率[(2.01±0.57)%]低于空白组[(3.21±0.74)%,t=2.813,P<0.05];perifosine组凋亡率[(12.38±3.01)%]高于空白组[(3.21±0.74)%,t=7.569,P<0.01]。SC79组每个视野透膜细胞数量(95.20±15.62)高于空白组(54.80±9.13,t=4.993,P<0.05);perifosine组每个视野透膜细胞数量(30.40±8.41)低于空白组(54.80±9.13,t=4.395,P<0.01)。结论激活Akt/MDM2/p53信号通路可促进NSCLC细胞增殖、侵袭,抑制其凋亡。Objective To observe the effect of regulating the protein kinase B(Akt)/mouse double minute 2(MDM2)/p53 signaling pathway on the malignant biological behavior of non-small cell lung cancer(NSCLC).Methods Logarithmic phase H1975 cell lines(purchased from the U.S.Type Culture Preservation Center)were treated with the Akt/MDM2/p53 signaling pathway activator SC79(4μg/ml)and inhibitor perifosine(5.0μmol/L),designated as the SC79 group and perifosine group,respectively.Cells from the untreated H1975 line were used as the control group.The H1975 cell line without any treatment was set as blank group.Cell proliferation,apoptosis rate and invasion ability were detected after 24 h intervention.One-way ANOVA was used for comparison among multiple groups and LSD-t test was used for comparison between two groups.Results The absorbance values at 24,48 and 72 h in SC79 group(0.38±0.05,0.63±0.07,0.89±0.12)were higher than those in blank group(0.25±0.04,0.46±0.07,0.62±0.11,t=4.540,3.840,3.709,P<0.01).The absorbance values in perifosine group at 24,48 and 72 h(0.16±0.04,0.29±0.06,0.40±0.09)were lower than those in blank group(0.25±0.04,0.46±0.07,0.62±0.11,t=3.558,4.123,3.461,P<0.01).The apoptosis rate[(2.01±0.57)%]in SC79 group was lower than that in blank group[(3.21±0.74)%,t=2.813,P<0.05].The apoptosis rate in perifosine group[(12.38±3.01)%]was higher than that in blank group[(3.21±0.74)%,t=7.569,P<0.01].The number of transmembrane cells per field in SC79 group(95.20±15.62)was greater than that in blank group(54.80±9.13,t=4.993,P<0.05).The number of cells in perifosine group(30.40±8.41)was less than that in blank group(54.80±9.13,t=4.395,P<0.01).Conclusion Activation of Akt/MDM2/p53 signaling pathway can promote proliferation and invasion,and inhibit apoptosis of NSCLC cells.

关 键 词：蛋白激酶B 双微体基因2 非小细胞肺癌 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]