长链非编码RNA父系表达遗传印记基因10靶向微小RNA-34a对口腔鳞状细胞癌生物学功能的影响  

Impact of long non-coding RNA paternally expressed 10 targeting microRNA-34a on the biological functions of oral squamous cell carcinoma

作　　者：路武豪[1] 张鹏[1] 高佩[1] 张艳飞[1] 冯龙[2] Lu Wuhao;Zhang Peng;Gao Pei;Zhang Yanfei;Feng Long(Department of Otolaryngology-Head and Neck Surgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China;Henan University of Chinese Medicine School of Medicine,Zhengzhou 450046,China)

机构地区：[1]郑州大学第一附属医院头颈外科,郑州450052 [2]河南中医药大学医学院,郑州450046

出　　处：《中华实验外科杂志》2024年第6期1257-1260,共4页Chinese Journal of Experimental Surgery

基　　金：河南省高校重点科研项目(18B310029)。

摘　　要：目的探讨长链非编码RNA父系表达遗传印记基因10(lncRNA PEG10)在口腔鳞状细胞癌(OSCC)中的表达,以及通过调节微小RNA(miR)-34a对OSCC细胞增殖和迁移的影响。方法收集53例口腔鳞癌组织和癌旁组织作为研究对象,采用实时定量反转录聚合酶链反应(RT-qPCR)分析组织中lncRNA PEG10和miR-34a的表达水平。口腔鳞癌SCC-25细胞采用随机数表法分为si-NC组、si-lncRNA EG10组、miR-34a mimics组、miRNA-NC组。采用细胞计数试剂盒(CCK-8)、细胞划痕实验和Transwell实验检测各组细胞增殖和迁移能力;双荧光素酶实验报告基因实验验证lncRNA PEG10与miR-34a的靶向关系。计量数据组间比较采用独立样本t检验,计数数据采用皮尔森卡方检验。结果lncRNA PEG10在OSCC肿瘤组织中的平均表达量(2.452±0.750)高于癌旁组织(1.293±0.326),差异有统计学意义(t=8.365,P<0.05)。lncRNA PEG10组细胞lncRNA PEG10表达水平(2.868±0.115)高于lncRNA对照组(0.973±0.003),差异有统计学意义(t=11.99,P<0.01)。miR-34a mimics组细胞miR-34a表达水平(1.310±0.010)高于miRNA对照组(0.251±0.020),差异有统计学意义(t=34.16,P<0.01)。lncRNA PEG10的高表达与淋巴结转移(χ^(2)=5.577,P<0.05)和临床分期(χ^(2)=6.606,P<0.05)相关。lncRNA PEG10沉默后,SCC-25细胞的增殖能力在48 h(0.416±0.009)和72 h(0.555±0.048)的检测点A值明显低于对照组(0.561±0.037和0.894±0.025,F_(48 h)=590.1,F_(72 h)=105.8,P<0.05)。lncRNA PEG10敲减组细胞划痕愈合率[(157.000±18.457)%]明显低于lncRNA对照组[(74.000±14.256)%],差异有统计学意义(t=19.452,P<0.05)。结论lncRNA PEG10在口腔鳞癌中表达上调,而miR-34a表达水平下调,lncRNA PEG10通过靶向结合miR-34a调节口腔鳞癌细胞增殖和迁移。Objective To investigate the expression of long non-coding RNA PEG10(lncRNA PEG10)in oral squamous cell carcinoma(OSCC)and its effect on OSCC cell proliferation and migration by regulating miR-34a.Methods A total of 53 OSCC tissues and paracancerous tissues were collected as study subjects,and the expression levels of lncRNA PEG10 and miR-34a in the tissues were analyzed by RT-qPCR.SCC-25 cells were divided into si-NC group,si-lncRNA PEG10 group,miR-34a mimics group,and miRNA-NC group by a random grouping method.Cell counting kit(CCK-8)assay,cell scratch assay and Transwell assay were used to detect the proliferation and migration ability of cells in each group.Dual luciferase reporter gene assay was used to verify the targeting relationship between lncRNA PEG10 and miR-34a.Continuous data were compared using independent samples t-test,while categorical data were analyzed with Pearson’s chi-square test.Results The mean expression of lncRNA PEG10 in OSCC tissues(2.452±0.750)was significantly higher than that in paracancerous tissues(1.293±0.326,t=8.365,P<0.05).The lncRNA PEG10 expression level in the lncRNA PEG10 group(2.868±0.115)was significantly higher than that in the lncRNA control group(0.973±0.003,t=11.99,P<0.001).The miR-34a expression level in the mimics group(1.310±0.010)was significantly higher than that in the miRNA control group(0.251±0.020,t=34.16,P<0.001).High expression of lncRNA PEG10 was correlated with lymph node metastasis(χ^(2)=5.577,P<0.05)and clinical stage(χ^(2)=6.606,P<0.05).The lncRNA PEG10 silencing was associated with the proliferative capacity of SCC-25 cells at 48 h(0.416±0.009)and 72 h(0.555±0.048).The absorbance values of the detection points were significantly lower than those in the control group(0.561±0.037 and 0.894±0.025,F_(48 h)=590.1,F_(72 h)=105.8,P<0.05).The cell scratch healing rate in the lncRNA PEG10 knockdown group[(157.000±18.457)%]was significantly lower than that in the lncRNA control group[(74.000±14.256)%,t=19.452,P<0.05].Conclusion lncRNA PEG10 exp

关 键 词：口腔鳞状细胞癌 长非编码RNA 长非编码RNA父系表达遗传印记基因10 微小RNA 

分 类 号：R739.8[医药卫生—肿瘤]