胃癌前病变大鼠模型胃黏膜细胞凋亡及表皮生长因子受体、神经丝蛋白200、表面抗原分化因子受体2蛋白表达的意义  

Apoptosis of gastric mucosa cells and expression of epidermal growth factor receptor, neurofilament protein 200, human epithelial growth factor receptor-2 protein in precancerous rats

作　　者：高峰[1] 谢燕兵[1] 马剑方[1] 师礼鹏[1] 史文彬 Gao Feng;Xie Yanbing;Ma Jianfang;Shi Lipeng;Shi Wenbin(Department of General Surgery,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第二医院普通外科,太原030001

出　　处：《中华实验外科杂志》2024年第6期1274-1277,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨胃癌前病变大鼠模型胃黏膜细胞凋亡及表皮生长因子受体(EGFR),神经丝蛋白200(NF-200),表面抗原分化因子受体2(C-erb-2)蛋白表达的意义。方法60只Wistar大鼠通过随机数表法分作模型组和对照组,每组各30只。模型组采用甲基硝基亚硝基胍干预90 d,建立胃癌前病变大鼠模型。对照组予以同等剂量的蒸馏水灌胃处理。分析两组大鼠胃黏膜上皮细胞凋亡率,胃黏膜EGFR、NF-200及C-erb-2蛋白表达情况,并以Pearson相关性分析。此外,通过受试者工作特征(ROC)曲线明确EGFR、NF-200及C-erb-2蛋白诊断胃癌前病变的效能。计量资料组间比较采用成组t检验。结果模型组大鼠胃黏膜上皮细胞凋亡率明显高于对照组[(37.22±0.49)%比(1.12±0.03)%],差异有统计学意义(t=402.772,P<0.05)。模型组大鼠胃黏膜EGFR、C-erb-2蛋白表达水平高于对照组(278.31±32.84、86.03±7.15比181.37±21.34、33.05±5.32),而NF-200蛋白表达水平低于对照组(0.71±0.03比1.00±0.00),差异均有统计学意义(t=13.557、52.947、32.561,P<0.05)。经Pearson相关性分析可得:胃癌前病变大鼠胃黏膜细胞凋亡率和EGFR、C-erb-2蛋白表达水平均呈正相关(r=0.623、0.605,P<0.05),与NF-200蛋白表达水平呈负相关(r=-0.617,P<0.05)。结论胃癌前病变大鼠模型胃黏膜细胞凋亡明显,且联合检测EGFR、C-erb-2及NF-200蛋白表达有助于胃癌前病变的早期诊断。Objective To investigate the apoptosis of gastric mucosa cells and the expression of epidermal growth factor receptor(EGFR),neurofilament protein 200(NF-200)and human epithelial growth factor receptor-2(C-ERB-2)in rat model of gastric precancerous lesion.Methods Totally,60 healthy Wistar rats were randomly divided into model and control groups,with 30 animals in each group.Model group was treated with methylnnitroguanidine for 90 days to establish a rat model of pregastric cancer.Control group was treated with the same dose of distilled water of gavage.The apoptosis rate of gastric epithelial cells and the protein expression levels of EGFR,NF-200 and C-ERB-2 in gastric mucosa of rats in the two groups were analyzed.Pearson correlation analysis was performed.In addition,the efficacy of EGFR,NF-200 and C-ERB-2 proteins in diagnosis of gastric precancerous lesions was determined by the receiver operating characteristic(ROC)curve analysis.Group t test was used to compare the measurement data between groups.Results The apoptosis rate of gastric mucosal epithelial cells in model group was(37.22±0.49)%,which was higher than in control group[(1.12±0.03)%,P<0.05].The expression levels of EGFR and C-ERB-2 in gastric mucosa of the model group were(278.31±32.84)and(86.03±7.15),which were higher than control group[(181.37±21.34),(33.05±5.32)],while the expression level of NF-200 was(0.71±0.03),which was lower than control group(1.00±0.00,all P<0.05).Pearson correlation analysis showed apoptosis rate of gastric mucosa cells was positively correlated with the protein expression levels of EGFR and C-ERB-2,and negatively correlated with the protein expression level of NF-200(all P<0.05).Conclusion Gastric mucosal cells in rat model of gastric precancerous lesions have obvious apoptosis,and the expression of EGFR and C-ERB-2 proteins is significantly increased,and the expression of NF-200 protein is significantly decreased.The combined detection of the expression of the above three proteins is helpful for the early diagnos

关 键 词：胃癌前病变 胃黏膜细胞 表皮生长因子受体 神经丝蛋白200 表面抗原分化因子受体2 

分 类 号：R735.2[医药卫生—肿瘤] R-332[医药卫生—临床医学]