三结构域蛋白59、核因子抑制蛋白在胃癌组织中表达及其与患者化疗效果和预后的关系  

Expression of triple domain protein 59 and nuclear factor inhibitory protein in gastric cancer tissue and their relationship with chemotherapy efficacy and prognosis in patients

作　　者：何春华[1] 胡毕文[1] 冯联忠[1] He Chunhua;Hu Biwen;Feng Lianzhong(Department of Anorectal Surgery,the Second Affiliated Hospital of Jiaxing University,Jiaxing 314000,China)

机构地区：[1]嘉兴学院附属第二医院胃肠外科,嘉兴314000

出　　处：《中华实验外科杂志》2024年第6期1278-1282,共5页Chinese Journal of Experimental Surgery

基　　金：浙江省医药卫生科技计划项目(2021KY1118);嘉兴市科技计划项目(2022AY30030);嘉兴市科技计划项目(2022AD30034)。

摘　　要：目的检测胃癌组织中三结构域蛋白59(TRIM59)、核因子抑制蛋白(IκBα)表达情况,并分析两者与患者化疗效果和预后的关系。方法选取2020年1月至2023年1月于嘉兴学院附属第二医院行手术治疗的胃癌患者103例,选取手术切除的距离癌组织边缘>2 cm的癌旁组织作为对照。比较胃癌组织、癌旁组织TRIM59、IκBα表达。经Pearson相关性分析法分析胃癌组织中TRIM59、IκBα表达的相关性。所有患者化疗3个周期后根据化疗效果分为耐药组、敏感组。比较耐药组、敏感组一般资料及胃癌组织TRIM59、IκBα表达。经Logistic回归模型分析胃癌化疗效果的影响因素;绘制Kaplan-Meier曲线分析胃癌组织TRIM59、IκBα表达与胃癌进展及生存的关系。计数资料表示为[例(%)],用χ^(2)检验;K-S检验符合正态分布的计量资料以均数±标准差(x±s)表示,组间比较用独立样本t检验。结果免疫荧光实验显示,TRIM59和IκBα可在相同的细胞区域内观察到两种不同的荧光信号,表现为细胞核内的共定位;胃癌组织的TRIM59表达水平高于癌旁组织(17.39±4.04比8.35±2.05,t=20.251,P<0.01);胃癌组织的IκBα表达水平高于癌旁组织(15.40±3.82比7.16±1.97,t=19.457,P<0.01)。Pearson相关性分析显示,胃癌组织中TRIM59、IκBα表达呈正相关(r=0.341,P<0.01);Logistic回归分析显示,年龄、TNM分期Ⅲ/Ⅳ期、未/低分化、TRIM59表达、IκBα表达是胃癌患者化疗效果的影响因素(P<0.05);耐药组胃癌组织的TRIM59表达水平高于敏感组组织(19.35±3.47比14.42±2.96,t=7.473,P<0.01);耐药组胃癌组织的IκBα表达水平高于敏感组组织(17.21±3.19比12.65±3.04,t=7.234,P<0.01)。103例胃癌患者失访15例,其余88例随访8~39个月,将胃癌组织中TRIM59、IκBα表达高于中位值的分为高TRIM59表达、高IκBα表达,低于中位值的分为低TRIM59表达、低IκBα表达。高TRIM59表达的中位无进展生存期(PFS)短于低TRIM59表达[24(22,2Objective To detect the expression of tripartite motif-containing protein 59(TRIM59)and nuclear factor inhibitor protein(IκBα)in gastric cancer tissues,and to analyze their relationship with the efficacy of chemotherapy and prognosis in patients.Methods A total of 103 patients with gastric cancer who underwent surgical treatment from January 2020 to January 2023 were selected,and the adjacent tissues resected more than 2 cm away from the cancerous tissues were selected as controls.The expression of TRIM59 and IκBαin gastric cancer tissues and adjacent tissues was compared.Pearson correlation analysis was used to analyze the correlation between TRIM59 and IκBαexpression in gastric cancer tissues.All patients were divided into drug-resistant group and sensitive group according to the efficacy of chemotherapy after 3 cycles of treatment.The general data and the expression of TRIM59 and IκBαin gastric cancer tissues were compared between the drug-resistant group and the sensitive group.Logistic regression model was used to analyze the influencing factors of the efficacy of gastric cancer chemotherapy.Kaplan-Meier curve was drawn to analyze the relationship between the expression of TRIM59 and IκBαin gastric cancer tissues and the progression and survival of gastric cancer.Count data is represented as[n(%)]and tested using a chi square test;The K-S test is used to represent econometric data that conforms to a normal distribution as mean±standard deviation(x±s),and independent sample t-test is used for inter group comparison.Results Immunofluorescence experiments showed that TRIM59 and IκBαcould be detected in the same cellular regions with two different fluorescence signals,representing co-localization in the nucleus.Compared with adjacent tissues,the fluorescence intensity of TRIM59 and IκBαin gastric cancer tissues was increased(P<0.05).The expression level of TRIM59 in gastric cancer tissue was higher than that in adjacent tissues(17.39±4.04 vs.8.35±2.05,t=20.251,P<0.01).The expression level of I�

关 键 词：胃癌 三结构域蛋白59 核因子抑制蛋白 化疗 预后 

分 类 号：R735.2[医药卫生—肿瘤]