细胞程序性死亡因子10调控蛋白磷酸化酶2A/p38信号通路介导胶质瘤细胞的迁移  

Programmed cell death 10 mediates the migration of glioma cells by regulating the PP2A/p38 signaling pathway

作　　者：万学焱 谢家钊 赵恺[1] 孔繁丽 梅齐 WAN Xueyan;XIE Jiazhao;ZHAO Kai;KONG Fanli;MEI Qi(不详;Department of Neurosurgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院神经外科,武汉430030 [2]华中科技大学同济医学院附属同济医院肿瘤中心,武汉430030 [3]华中科技大学同济医学院基础医学院病理生理学系 [4]武汉儿童医院麻醉科

出　　处：《临床外科杂志》2024年第7期693-696,共4页Journal of Clinical Surgery

基　　金：湖北省自然科学基金面上项目(2021CFB394);华中科技大学同济医学院附属同济医院留学归国人员启动基金(2020HGRY009);武汉市卫健委研究项目(WX20Q35)。

摘　　要：目的探讨细胞程序性死亡因子10(programmed cell death 10,PDCD10)介导胶质瘤细胞迁移的分子机制。方法siRNA构建siPDCD10胶质瘤细胞系(U251),同时采用蛋白磷酸化酶2A(protein phosphatase 2A,PP2A)抑制剂冈田酸(Okadaic acid,OA)处理细胞,研究下调PDCD10对胶质瘤细胞行为的影响;蛋白质印迹法(western blot,WB)分别检测对照组和PDCD10低表达组胶质瘤细胞PDCD10、PP2A,PP2Ac-307,p38和pP38的表达,研究PDCD10调控PP2A/p38信号的分子机制。结果下调胶质瘤细胞PDCD10促进肿瘤细胞的迁移(P<0.05),而这一效应可被PP2A抑制剂OA抑制(P<0.01)。进一步研究显示,siPDCD10通过降低PP2A的磷酸化水平,进而升高p38的活性,促进胶质瘤细胞的迁移(P<0.01)。结论PDCD10可能通过调控PP2A/p38信号介导胶质瘤细胞的迁移。Objective To explore the molecular mechanism of programmed cell death 10(PDCD10)mediating glioma migration.Methods A PDCD10-silenced glioma cell line(U251)was constructed by siRNA,and the cells were treated with Okadaic Acid(OA),a protein phosphatase 2A(PP2A)phosphatase inhibitor,to investigate the effect of down-regulation of PDCD10 on the behavior of U251 cells;Western Blot(WB)was applied to detect the expression of PDCD10,PP2A,PP2Ac-307,p38 and pP38 in the control group and PDCD10 down-regulation group of U251 cells,to study the mechanism of PDCD10 regulation of PP2A/p38 signaling.Results Down-regulation of PDCD10 in U251 cells promoted the cells migration(P<0.05),which could be inhibited by PP2A phosphatase inhibitor OA(P<0.01).Further studies showed that down-regulation of PDCD10 promoted glioma cell migration via modulating the phosphorylation of PP2A,which in turn altered the activity of p38(P<0.01).Conclusion PDCD10 may mediate glioma cell migration by regulating PP2A/p38 signaling.

关 键 词：细胞程序性死亡因子10 胶质瘤 迁移 PP2A/p38 

分 类 号：R739.41[医药卫生—肿瘤]