高静压酶解法制备猪小肠肝素及其稳定性研究  

Preparation of Porcine Small Intestinal Heparin by High Hydrostatic Pressure Enzymatic Hydrolysis and Its Stability

作　　者：吴晓龙 王钰堡 刘容旭 刘丹怡 韩建春[1,2] 管凤伟 李新 韩帮伟 WU Xiaolong;WANG Yubao;LIU Rongxu;LIU Danyi;HAN Jianchun;GUAN Fengwei;LI Xin;HAN Bangwei(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Institute of Green Food Science,Harbin 150028,China;Heilongjiang Hanheng Environmental Protection Technology Co.,Ltd.,Harbin 150028,China;Fengrun Biotechnology Incorporated Company,Harbin 150036,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028 [3]黑龙江翰恒环保科技有限公司,黑龙江哈尔滨150028 [4]丰润生物科技股份有限公司,黑龙江哈尔滨150036

出　　处：《食品工业科技》2024年第16期209-219,共11页Science and Technology of Food Industry

基　　金：黑龙江省重点研发计划:家畜小肠高值化加工关键技术开发及产业化示范(GA21B015);马铃薯淀粉加工副产物资源化技术研发(SKY20210601)。

摘　　要：本研究以猪小肠粘膜为原料,通过响应面试验优化高静压酶解法提取肝素(Heparin,Hep)的最佳工艺,研究大孔树脂纯化Hep吸附条件,并探讨Hep的稳定性(pH、温度、光照)。结果表明:最佳酶解工艺为选取碱性蛋白酶,时间3.1 h,温度55℃,pH9.5,压力100 MPa,酶底物比9%,盐浓度2.0%,测得Hep实际平均浓度为11.56±0.12 U/mL;利用D958树脂纯化Hep,上样流速1.5 BV/h,洗脱液浓度4 mol/L NaCl,洗脱流速1.5 BV/h,纯化后Hep纯度可达68.75%,即达到电泳纯;在稳定性研究中,Hep在pH为7.5时稳定性最好,温度在40℃时具有较好稳定性,光照对Hep稳定性没有影响。本研究可大幅度提升Hep的提取效果,为猪小肠高值化加工利用提供理论和技术支持。In this study,the mucosa of porcine small intestine was used as the raw material to optimize the optimal process for the extraction of heparin(Hep)by high hydrostatic pressure enzymatic hydrolysis by response surface test,to study the adsorption conditions of Hep purified by macroporous resin,and to explore the stability of Hep(pH,temperature,and light).The results showed that the optimal enzymatic process was alkaline protease,3.1 h,55℃,pH9.5,pressure 100 MPa,enzyme addition 9%,salt concentration 2.0%,the actual average concentration of Hep was measured to be 11.56±0.12 U/mL.The purification of Hep was carried out by using the D958 resin,with the up-sampling flow rate of 1.5 BV/h,the concentration of the eluent of 4 mol/L NaCl,and the elution flow rate of 1.5 BV/h,the purity of Hep could reach 68.75%after purification,and the electrophoretic purity achieved.In the stability study,Hep had the best stability at pH7.5,better stability at 40℃,and light had no effect on the stability of Hep.This study can greatly improve the extraction effect of Hep and provide theoretical and technical support for the high value processing and utilization of pig small intestine.

关 键 词：猪小肠 高静压酶解 肝素 大孔树脂 稳定性 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]