显著琼娜蟹(Jonas distinctus)线粒体基因组全序列测定及系统发育分析  

THE COMPLETE MITOCHONDRIAL GENOME OF JONAS DISTINCTUS(BRACHYURA:CORYSTIDAE)AND ITS PHYLOGENETIC STATUS

作　　者：张楠楠 汪凯欣 吴文超 吕文拯 吕振明 龚理 ZHANG Nan-Nan;WANG Kai-Xin;WU Wen-Chao;LYU Wen-Zheng;LYU Zhen-Ming;GONG Li(Zhejiang Ocean University,Marine Science and Technology College,Zhoushan 316022,China;National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院,浙江舟山316022 [2]海洋生物种质资源发掘利用国家地方联合工程研究中心,浙江舟山316022

出　　处：《海洋与湖沼》2024年第4期997-1007,共11页Oceanologia Et Limnologia Sinica

基　　金：浙江省自然科学基金项目,LY21C190007号;舟山市科技局资助项目,2021C21007号。

摘　　要：短尾次目(Brachyura)物种蟹化现象是节肢动物进化史上十分重要的形态创新,但是也有少数种类在形态上表现出显著的去蟹化。为解析短尾次目去蟹化典型代表盔蟹总科(Corystoidea)物种的进化地位,以盔蟹科(Corystidae)显著琼娜蟹(Jonas distinctus)为对象,测定分析其线粒体基因组全序列特征,并基于13个蛋白质编码基因序列,构建短尾次目系统发育树。结果表明,显著琼娜蟹线粒体基因组全长为16038 bp,包含37个编码基因和一段长非编码区;与短尾次目祖先型线粒体基因排序相比,该线粒体基因组发生了明显重排,其控制区、ND2及6个tRNA基因(I、Q、M、W、C和Y)的相对位置与当前已报道的短尾次目线粒体基因排序不同,表现出一种全新的基因排序,并可用复制-随机丢失模型解释该重排现象;两种方法所得到系统发育树拓扑结构一致,均显示盔蟹科与菱蟹科(Parthenopidae)亲缘关系最近,而与同样具有去蟹化性状的蛙蟹总科(Raninoidea)(如蛙蟹科Raninidae和琵琶蟹科Lyreididae)关系较远,表明短尾次目独特的去蟹化现象经历了多次独立演化。显著琼娜蟹的线粒体基因组分析结果不仅丰富了短尾次目线粒体基因重排类型,同时也揭示了盔蟹科在短尾次目中系统发育关系,为更好地厘清短尾次目分类及其起源与演化提供了新的数据支撑和理论依据。Carcinization is a significant morphological innovation in the evolutionary history of arthropods,particularly within Brachyura.However,few species own apparent decarcinization traits,deviating significantly from the typical crab morphology.Corystoidea species serve as a representative example of decarcinization in Brachyura,while the evolutionary status of this group is still unclear.The complete mitochondrial genome(mitogenome)can provide important molecular information for revealing phylogenetic relationships.The complete mitogenome of Jonas distinctus(Brachyura,Corystidae)was sequenced and analyzed.The 16038-bp mitogenome contains the entire set of 37 genes and a long non-coding region(CR).Compared to the ancestral mitogenomes of Brachyura,this mitogenome exhibits significant gene rearrangements.The gene order of CR,ND2,and six tRNA genes(I,Q,M,W,C,and Y)differed from those that have reported,indicating a completely new type of gene arrangement.The phylogenetic relationships based on 13 PCGs were constructed.Both Bayesian inference(BI)and maximum likelihood(ML)trees demonstrate that Corystidae is the closest relative to Parthenopidae.Conversely,the Raninoidea group(e.g,Raninidae and Lyreididae),capturing the same decarcinization traits,had a relatively distant relationship with Corystidae.This phylogenetic relationship indicates that the unique decarcinization phenomenon in Brachyura species had evolved independently several times.This study enhanced our understanding of mitochondrial gene rearrangement and revealed the evolutionary position of Corystidae among Brachyura,as well as provided data for the classification and evolutionary origin of Brachyura.

关 键 词：短尾次目 盔蟹科 线粒体基因组 基因重排 复制-随机丢失 系统进化 

分 类 号：Q789[生物学—分子生物学] Q954Q959.22