直接酯化法合成月桂酸单甘油酯及对金黄色葡萄球菌的抑菌性能研究  

Synthesis of glycerol monolaurate by direct esterification andits antibacterial activity against Staphylococcus aureus

作　　者：张轩 杨婧 ZHANG Xuan;YANG Jing(College of Environmental Science and Engineering,Shaanxi University of Science and Technology,Xi’an 710021,China)

机构地区：[1]陕西科技大学环境科学与工程学院,西安710021

出　　处：《功能材料》2024年第7期7015-7021,共7页Journal of Functional Materials

基　　金：国家自然科学基金青年科学基金项目(32000295)。

摘　　要：选择月桂酸和甘油为原料、对甲苯磺酸为催化剂,通过单因素试验测定了反应温度、反应时间、搅拌速度、原料摩尔比、催化剂用量等对月桂酸单甘油酯(GML)纯度的影响,研究了GML浓度和pH值变化对金黄色葡萄球菌的抑制效果。结果表明,在n(甘油)∶n(月桂酸)=3∶1,反应温度为180℃,反应时间为3.5 h,搅拌速度为250 r/min,对甲苯磺酸用量为0.4%(质量分数)条件下制备出的GML纯度最高,该工艺为最优合成工艺;正交试验测试表明,合成温度对GML纯度的影响最大,最优合成工艺下制备出的GML初始纯度为47.7%,提纯后纯度可达97.2%。抑菌性能测试表明,GML对金黄色葡萄球菌的抑制效果与其浓度呈正相关,当浓度达到12 mg/mL时,抑菌圈的直径达到了最大值16.1 mm;GML对金黄色葡萄球菌的抑菌率均随着时间的增加逐渐降低,在pH值=5.7和7.2的条件下,GML对金黄色葡萄球菌的抑菌半衰期分别为54和49 h,可见弱酸条件下GML对金黄色葡萄球菌的抑菌半衰期更大,抑菌效果更佳。Choosing lauric acid and glycerol as raw materials and p-toluene sulfonic acid as a catalyst,the effects of reaction temperature,reaction time,stirring speed,raw material molar ratio and catalyst dosage on the purity of glycerol monolaurate(GML)were determined through single factor test.And the inhibitory effect of GML concentration and pH value changes on Staphylococcus aureus were studied.The results showed that the highest purity of GML was obtained under the conditions of n(glycerol)∶n(lauric acid)=3∶1,reaction temperature of 180℃,reaction time of 3.5 h,stirring speed of 250 r/min,and p-toluenesulfonic acid dosage of 0.4 wt%.This process was the optimal synthesis process.The orthogonal experimental test showed that the synthesis temperature had the greatest impact on the purity of GML.The initial purity of GML prepared under the optimal synthesis process was 47.7%,and the purity after purification could reach 97.2%.The antibacterial performance test showed that the inhibitory effect of GML on Staphylococcus aureus was positively correlated with its concentration.When the concentration reached 12 mg/mL,the diameter of the antibacterial zone reached its maximum value of 16.1 mm.The antibacterial rates of GML against Staphylococcus aureus gradually decreased with time.Under pH values of 5.7 and 7.2,the half-lives of GML against Staphylococcus aureus were 54 and 49 hours,respectively.It can be seen that under weak acid conditions,GML has a longer antibacterial half-life and better antibacterial effect on Staphylococcus aureus.

关 键 词：月桂酸单甘油酯 最优工艺 单因素试验 金黄色葡萄球菌 抑菌性能 

分 类 号：TQ645.6[化学工程—精细化工] TQ22