基于广泛代谢组学解析不同品种李果实代谢物的差异  

Non-Targeted Metabolomics Analysis of Differences in Fruit Metabolites of Different Plum Varieties

作　　者：常晓晓[1] 彭程 陈慧琼 邱继水[1] 陆育生 CHANG Xiaoxiao;PENG Cheng;CHEN Huiqiong;QIU Jishui;LU Yusheng(Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs,Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院果树研究所,农业农村部南亚热带果树生物学与遗传资源利用重点实验室,广东省热带亚热带果树研究重点实验室,广东广州510640

出　　处：《食品科学》2024年第16期151-160,共10页Food Science

基　　金：广东省现代农业产业技术体系创新团队建设项目(2023KJ116);广东省落叶果树种质资源圃运行维护项目(2023-NBH-00-016)。

摘　　要：利用非靶向代谢组学对广东5个不同品种/系李果实进行代谢物分析,共检出951种代谢物,其中初级代谢物489种、次级代谢物315种。5个品种李果实初级代谢物中脂类含量最高,其次是糖类、醇类、氨基酸类、有机酸类,核苷酸和维生素含量相对较少;次级代谢物中多酚类化合物含量最高,其次是植物激素、生物碱和萜类,苯丙素和木脂素类含量较少。大蜜李果实中脂类含量最高的是十一烷酸,占脂类总量的92.9%;糖类含量在大蜜李果实中仅次于脂类,其中松二糖含量最高,其含量为蔗糖的9.5倍、葡萄糖的8倍及果糖的14.6倍,可能是其具有蜜味的主要贡献因子;初级代谢物种类最多的是氨基酸及其衍生物,共鉴定到170种;L-苹果酸是主要的有机酸组分,占总有机酸及其衍生物含量的57.1%;维生素类中VB6含量最高,占比达51.5%;多酚类化合物中含量最多的是类黄酮,占比达77.7%。对其余4个品种与大蜜李果实分别进行代谢物差异分析,结果表明:青皮李与大蜜李的差异代谢物84种;小蜜李与大蜜李的差异代谢物73种;红线李与大蜜李的差异代谢物113种;大果迟熟三华李与大蜜李的差异代谢物34种,对差异代谢物富集的代谢途径进行分析发现,4组的差异代谢物主要富集于氨基酸、类黄酮、花青素、生物碱等合成代谢途径。大果迟熟三华李与大蜜李差异代谢物主要有C-戊糖基-木犀草素-C-己糖苷、多巴胺、三叶苷等类黄酮和酚类物质,以及烟酰胺腺嘌呤二核苷酸、β-烟酰胺单核苷酸等核苷酸类,这些物质可能是二者出现成熟期差异的关键物质。A total of 951 metabolites including 489 primary ones and 315 secondary ones were detected in plum fruit from 5 different varieties/lines in Guangdong by non-targeted metabolomics.For all plum varieties/lines,lipids were the most abundant primary metabolites,followed by sugars,alcohols,amino acids and organic acids,while the contents of nucleotides and vitamins were relatively low.Polyphenols were the most abundant secondary metabolites,followed by plant hormones,alkaloids and terpenoids,while the contents of phenylpropanoids and lignan were very low.The most abundant lipid component in Dami plum was undecanoic acid,accounting for 92.9%of the total lipids.The content of sugar in the fruit of plum is second only to lipid.The most abundant sugar in Dami plum was turanose,9.5,8 and 14.6 times as abundant as sucrose,glucose and fructose,respectively,which might be the major contributor to the honey flavor in Dami plum.A total of 170 amino acids and their derivatives were identified,greater than that of any other primary metabolites.L-Malic acid was the major organic acid,accounting for 57.1%of the total organic acids and their derivatives.VB6 was the most abundant vitamin,accounting for 51.5%of the total vitamins.Flavonoids were the most abundant polyphenols,accounting for 77.7%of the total polyphenols.Altogether,84,73,113 and 34 differential metabolites were identified between Dami plum and Qingpi,Xiaomi,Hongxian and Daguochishu Sanhua plum,respectively.Metabolic pathway enrichment analysis of differential metabolites showed that the differential metabolites of the 4 groups were mainly enriched in amino acids,flavonoids,anthocyanins,alkaloids and synthetic and metabolic pathways.The major differential metabolites between Daguochishu Sanhua and Dami plum were flavonoids and phenols such as C-pentosyl-luteolin-C-hexoside,dopamine and trilobatin,and nucleotides such as nicotinamide adenine dinucleotide andβ-nicotinamide mononucleotide.These substances may be the key differential metabolites between the 2 varieties at

关 键 词：李 果实 代谢物 非靶向代谢组学 

分 类 号：S662.3[农业科学—果树学]