微原纤维相关蛋白3在调控胶质瘤干细胞间充质表型转化中的作用  

Role of microfibril-associated protein 3 in regulating mesenchymal transition of glioma stem cells

作　　者：郭姝画 樊扬 田风 王传新 杜鲁涛 李培龙 郭兴 徐硕[1,4] GUO Shuhua;FAN Yang;TIAN Feng;WANG Chuanxin;DU Lutao;LI Peilong;GUO Xing;XU Shuo(Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong,China;Department of Clinical Laboratory,The Second Hospital of Shandong University,Jinan 250033,Shandong,China;Department of Neurosurgery,The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan 250014,Shandong,China;Department of Neurosurgery,Qilu Hospital of Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]山东大学齐鲁医学院,山东济南250012 [2]山东大学第二医院检验医学中心,山东济南250033 [3]山东第一医科大学第一附属医院(山东省千佛山医院)神经外科,山东济南250014 [4]山东大学齐鲁医院神经外科,山东济南250012

出　　处：《山东大学学报（医学版）》2024年第6期9-16,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(82272413);泰山学者青年专家计划(tsqn201909174);国家重点研发计划(2022YFC2406404);山东省自然科学基金(ZR2023MH036)。

摘　　要：目的探讨微原纤维相关蛋白3(microfibril-associated protein 3,MFAP3)在胶质母细胞瘤(glioblastoma,GBM)中的表达与临床意义,以及对于胶质瘤干细胞(glioma stem cells,GSCs)恶性生物学行为的影响。方法利用TCGA数据和CGGA数据分析GBM中MFAP3表达情况及其与患者预后相关性;使用Western blotting检测胶质瘤干细胞系中MFAP3蛋白表达水平;探究敲减及过表达MFAP3对胶质瘤干细胞自我更新能力和表型的影响。结果MFAP3在GBM中高表达,且与患者不良预后相关。间充质型GSCs中MFAP3的表达水平高于前神经元型GSCs。MFAP3敲减后,GSCs自我更新能力减弱,间充质表型标志物CD44、YKL40蛋白水平均降低,过表达与之相反。结论MFAP3对GBM恶性生物学进展具有重要调控作用,有望成为新的GBM临床诊断标志物和治疗干预靶点。Objective To investigate the expression and clinical significance of microfibril-associated protein 3(MFAP3)in glioblastoma(GBM)and its influence on the malignant biological behavior of glioma stem cells(GSCs).Methods TCGA data and CGGA data were used to analyze the expression of MFAP3 in GBM and its correlation with patient prognosis.Western blotting was used to detect the protein expression level of MFAP3 in GSCs.The effects of MFAP3 knockdown and overexpression on the self-renewal ability and phenotype of GSCs were explored.Results MFAP3 was highly expressed in GBM and correlated with poor prognosis.The expression level of MFAP3 in mesenchymal GSCs was higher than that in preneuronal GSCs.After MFAP3 knockdown,the self-renewal ability of GSCs was weakened,and the protein levels of mesenchymal phenotype markers CD44 and YKL40 were decreased,whereas overexpression exhibited the opposite trends.Conclusion MFAP3 plays an important role in regulating the malignant biological progression of GBM and is expected to be a new clinical diagnostic marker and therapeutic intervention target for GBM.

关 键 词：微原纤维相关蛋白3 胶质瘤干细胞 表型转化 自我更新 预后 

分 类 号：R739.41[医药卫生—肿瘤]