中药药用成分β-谷甾醇的预测及其对辐照宫颈癌细胞增殖与迁移的影响  

Prediction ofβ-sitosterol as medicinal component in traditional Chinese medicine and its effects on the proliferation and migration of irradiated cervical cancer cells

作　　者：王含梦 胡煜 陈然众 彭昌民 肖媛元 唐双阳 李乐 WANG Hanmeng;HU Yu;CHEN Ranzhong;PENG Changmin;XIAO Yuanyuan;TANG Shuangyang;LI Le(Pathogenic Biology Institute,School of Basic Medicine,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;School of Public Health,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学衡阳医学院基础医学院病原生物学研究所,湖南衡阳421001 [2]南华大学衡阳医学院公共卫生学院,湖南衡阳421001

出　　处：《中南医学科学杂志》2024年第4期506-511,共6页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81402169);湖南省教育厅重点项目(23A0328)。

摘　　要：目的预测宫颈癌差异基因的靶向中药关键药用成分,并分析其对辐照宫颈癌细胞增殖与迁移的影响。方法通过Coremine Medical、TCMSP、Uniprot以及Cytoscape数据库查找宫颈癌差异基因靶向中药及其药用成分和对应靶点,并筛选出核心靶点和关键药用成分。通过STRING数据库分析关键药用成分对应靶点基因与宫颈癌差异基因的蛋白互作。采用MTT、细胞划痕实验和Western blotting实验检测关键药用成分对辐照前后宫颈癌HeLa、Siha细胞增殖、迁移和DNA断裂水平的影响。结果共预测出宫颈癌差异基因的有效靶向中药9种。共得到96种药用成分和252个靶点基因,其中关键药用成分为β-谷甾醇,并筛选出其对应的核心靶点基因10个。β-谷甾醇的相关核心靶点基因与宫颈癌差异基因表达蛋白存在互作。β-谷甾醇抑制辐照前后宫颈癌细胞的增殖与迁移,促进宫颈癌HeLa细胞以及Siha细胞的DNA断裂水平。结论预测宫颈癌差异基因的靶向中药关键药用成分为β-谷甾醇,其可抑制辐照宫颈癌细胞的增殖与迁移。Aim To predict the targeted key medicinal components of traditional Chinese medicine for differentially expressed genes in cervical cancer,and analyze their effects on the proliferation and migration of irradiated cervical cancer cells.Methods Differentially-targeted traditional Chinese medicine as well as its medicinal components and corresponding targets for cervical cancer were searched through Coremine Medical,TCMSP,Uniprot,and Cytoscape databases,and core targets and key medicinal components were screened.The protein interactions between target genes corresponding to key medicinal ingredients and differentially expressed genes in cervical cancer were analyzed through the STRING database.The effects of key medicinal ingredients on the proliferation and migration,deoxyribonucleic acid(DNA)breakage level of cervical cancer HeLa,Siha cells were detected by using MTT,scratch tests and Western blotting.Results 9 effectively targeted traditional Chinese medicines have been predicted to differentially expressed genes in cervical cancer.96 medicinal ingredients and 252 target genes were obtained,among which the key medicinal ingredient wasβ-sitosterol and 10 corresponding core target genes were screened out.There was the protein interaction between the core targeted-genes ofβ-sitosterol and the differential-genes of cervical cancer.β-sitosterol inhibited the proliferation and migration of cervical cancer cells before and after irradiation,promoted DNA fragmentation levels in HeLa cells of cervical cancer and Siha cells.Conclusion The key medicinal component of targeted traditional Chinese medicine for predicting differentially expressed genes in cervical cancer wasβ-sitosterol,which could inhibit the proliferation and migration of irradiated cervical cancer cells.

关 键 词：宫颈癌 药用成分 Β-谷甾醇 细胞增殖 辐射敏感性 细胞迁移 中药 HeLa细胞 SIHA细胞 

分 类 号：R737.33[医药卫生—肿瘤]