能够诱导神经元hnRNPA1蛋白发生核-质易位方法的比较  

Comparison of Methods that Lead to Changes in the Nuclear and Cytoplasmic Positions of hnRNPA1 Proteins in Neurons

作　　者：郭春艳[1,2] 靳占奎 封青[1,2] 闫莉婷[1,2] 冯杨萌 徐翠香 GUO Chunyan;JIN Zhankui;FENG Qing;YAN Liting;FENG Yangmeng;XU Cuixiang(Key Laboratory of Infection and Immune Diseases,Shaanxi Provincial People's Hospital,Xi'an 710068,China;Research Center of Cell Immunological Engineering and Technology of Shaanxi Province,Xi'an 710068,China;Department of Orthopedics,Shaanxi Provincial People's Hospital,Xi'an 710068,China)

机构地区：[1]陕西省人民医院感染与免疫疾病重点实验室,陕西西安710068 [2]陕西省细胞免疫工程技术研究中心,陕西西安710068 [3]陕西省人民医院骨科,陕西西安710068

出　　处：《药物生物技术》2024年第3期228-231,共4页Pharmaceutical Biotechnology

基　　金：陕西省重点研发计划一般项目(No.2024SF-YBXM-156);陕西省自然科学基础研究计划项目(No.2023-JC-QN-0855)。

摘　　要：神经退行性病变是多发性硬化(MS)疾病进展的重要特征,异质核糖核蛋白A1(hnRNPA1)的功能障碍已被证明与神经退行性疾病的发病机制有关。临床研究发现,甲型流感病毒感染可促进MS患者病情加重,但具体机制不明。实验通过探讨影响hnRNPA1蛋白发生核质易位的可能因素,为MS的发生机制提供基础数据。首先培养人神经细胞SHSY5Y,然后检测该细胞中是否有hnRNPA1基因的表达;其次用细胞因子IL-17、TNF-α和IFN-γ及甲型流感病毒诱导机体产生的抗人hnRNPA1蛋白的自身抗体H1-84处理神经细胞;最后检测hnRNPA1蛋白的定位是否发生了变化。结果显示,用不同浓度的IFN-γ及抗人hnRNPA1蛋白的自身抗体H1-84均可引起SHSY5Y细胞中的hnRNPA1蛋白从细胞核转移到细胞质中,发生错误定位,功能失调的hnRNPA1可能在流感病毒感染促进MS疾病发生及发展过程中发挥了重要作用。Neurodegeneration was an important feature of the progression of multiple sclerosis(MS).Dysfunctions of heterogeneous nuclear ribonucleoprotein A1(hnRNPA1)had been shown to be associated with the pathogenesis of neurodegenerative diseases.Clinical studies had shown that influenza A virus infection can promote the aggravation of MS patients,but the specific mechanism was unknown.In neural cell lines,different concentrations of IFN-γand the autoantibodies against human hnRNPA1 protein induced by influenza A virus can cause the transfer of hnRNPA1 from the nucleus to the cytoplasm and mislocalization.The dysfunctional hnRNPA1 may play an important role in the development and progression of MS disease promoted by influenza virus infection.

关 键 词：不均一性核糖蛋白 细胞因子 干扰素 自身抗体 流感病毒 

分 类 号：R36[医药卫生—病理学]