海带、裙带菜和龙须菜凝集素提取及其性质研究  

Extraction and Properties of Lectins from Laminaria japonica,Undaria pinnatifida and Gracilaria lemaneiformis

作　　者：王珊珊 张瑞标 WANG Shanshan;ZHANG Ruibiao(Weihai Ocean Vocational College,Rongcheng Shandong 264300;Weihai Characteristic Fruits and Vegetables High Value Processing Engineering Technology Research Center,Rongcheng Shandong 264300;Marine Economic Algae Resources Development and Utilization Engineering Technology Collaborative Innovation Center of Shandong Province,Rongcheng Shandong 264300)

机构地区：[1]威海海洋职业学院,山东荣成264300 [2]威海市特色果蔬高值加工工程技术研究中心,山东荣成264300 [3]山东省海洋经济藻类资源开发与利用工程技术协同创新中心,山东荣成264300

出　　处：《现代农业科技》2024年第15期137-140,共4页Modern Agricultural Science and Technology

基　　金：威海市特色果蔬高值加工工程技术研究中心科研专项资金项目(GSGC2021-0002);山东省海洋经济藻类资源开发与利用工程技术协同创新中心项目(MADU20210002,MADU20210004);山东省高等职业教育高水平专业群建设项目“水产养殖技术专业群”(33)。

摘　　要：采用0.9%生理盐水、PBS(pH=7.2的磷酸盐缓冲液)和0.9%生理盐水+PBS的混合液3种提取液,提取海带、裙带菜和龙须菜凝集素,探究其凝集活性、蛋白浓度和比活力。结果表明:0.9%生理盐水提取海带凝集素的凝集活性、比活力皆最大,分别为0.062 U/μg(产生凝集现象的最小凝集素量)和16258.16 U/mg;0.9%生理盐水提取裙带菜凝集素的蛋白浓度最大,为14.98 mg/mL,海带次之。综合分析认为,不同提取液对海藻凝集素的凝集活性、蛋白浓度、比活力影响显著,0.9%生理盐水提取海藻凝集素效果较优。In this study,three extracting solutions,such as 0.9%physiological saline,PBS(phosphate buffer with pH=7.2)and mixture of 0.9%physiological saline+PBS,were used to extract lectin from Laminaria japonica,Undaria pinnatifida and Gracilaria lemaneiformis,and to investigate their agglutination activity,protein concentration and specific activity.The results showed that the agglutination activity and specific activity of Laminaria japonica lectin extracted by 0.9%physiological saline were the highest,which were 0.062 U/μg(the minimum lectin amount producing agglutination phenomenon)and 16258.16 U/mg,respectively.The maximum protein concentration of Undaria pinnatifida lectin extracted by 0.9%physiological saline was 14.98 mg/mL,followed by Laminaria japonica lectin.Comprehensive analysis showed that different extracting solutions had significant effects on the agglutination activity,protein concentration and specific activity of algal lectin.The extraction effect of algal lectin with 0.9%physiological saline was better.

关 键 词：海藻凝集素 凝集活性 蛋白浓度 比活力 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程]