乳酸菌肽聚糖对雏鸭饲粮中黄曲霉毒素B 1的脱毒效果研究  

Study on Detoxification Effects of Lactic Acid Bacteria Peptidoglycan on Aflatoxin B 1 of Ducklings’Diet

作　　者：邵怡豪 王煜琦 张宇豪 张广明 肖发沂 李庆梅 SHAO Yihao;WANG Yuqi;ZHANG Yuhao;ZHANG Guangming;XIAO Fayi;LI Qingmei(Department of Animal Science and Technology,Shandong Vocational Animal Science and Veterinary College,Weifang 261061,China;Muping Animal Disease Control Center,Yantai 264100,China)

机构地区：[1]山东畜牧兽医职业学院动物科技学院,潍坊261061 [2]烟台市牟平区动物疫病预防与控制中心,烟台264100

出　　处：《动物营养学报》2024年第7期4339-4349,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省现代农业产业技术体系创新团队建设项目(SDAIT-21-03);山东省新旧动能转换重大产业攻关项目(2021-50);山东省高等学校低碳绿色养殖新技术研发中心项目(2022)。

摘　　要：本试验旨在探究乳酸菌肽聚糖对黄曲霉毒素B_(1)(AFB_(1))的吸附作用,为乳酸菌肽聚糖的制备和饲粮中AFB_(1)的脱毒提供科学依据及理论基础。试验以4种乳酸菌(嗜酸乳杆菌、嗜热链球菌、罗伊氏乳杆菌和植物乳杆菌)为原料提取乳酸菌肽聚糖,采用高效液相色谱法比较4种乳酸菌肽聚糖对AFB_(1)的体外吸附率,选取体外吸附效果最好的罗伊氏乳杆菌肽聚糖进行动物试验。选择1日龄健康樱桃谷鸭120只,随机分为5组,每组4个重复,每个重复6只。对照组饲喂基础饲粮,AFB_(1)组在基础饲粮中添加0.10 mg/kg AFB_(1),试验组在AFB_(1)组饲粮中分别添加0.10%(Ⅰ组)、0.15%(Ⅱ组)和0.20%(Ⅲ组)罗伊氏乳杆菌肽聚糖。预试期3 d,正试期21 d。结果表明:1)乳酸菌肽聚糖种类、添加量及二者的交互作用均能够显著影响AFB_(1)的体外吸附率(P<0.05),其中10.0 mg/mL罗伊氏乳杆菌肽聚糖对AFB_(1)的体外吸附率最高,达75.29%。2)与对照组相比,AFB_(1)组的平均日增重、平均日采食量显著降低(P<0.05),料重比显著升高(P<0.05);血浆谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)活性及丙二醛(MDA)含量显著升高(P<0.05),肝脏、脾脏、胸腺和法氏囊指数显著升高(P<0.05),血浆免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC)显著降低(P<0.05)。3)与AFB_(1)组相比,Ⅰ、Ⅱ、Ⅲ组的平均日增重显著升高(P<0.05),料重比显著降低(P<0.05);Ⅰ、Ⅱ、Ⅲ组的血浆IgG、IFN-γ、IL-2、IL-4含量和SOD、GSH-Px活性显著升高(P<0.05),血浆AST、AKP、ALT活性及MDA含量显著降低(P<0.05);Ⅱ、Ⅲ组的胸腺和法氏囊指数显著降低(P<0.05)。由此可见,不同乳酸菌肽聚糖对AFB_(1)体外吸附效果不同,饲粮中添加罗伊氏乳杆菌肽聚糖能够部分消除AFB_(1)This experiment was conducted to explore the adsorption effect of lactic acid bacteria peptidoglycan on aflatoxin B_(1)(AFB_(1)),so as to provide a theoretical basis for the preparation of lactic acid bacteria peptidoglycan and the detoxification of AFB_(1) in diet.The experiment used 4 types of lactic acid bacteria(Lactobacillus acidophilus,Streptococcus thermophilus,Lactobacillus reuteri and Lactobacillus plantarum)as raw materials to extract lactic acid bacteria peptidoglycan,the in vitro adsorption rates of 4 types of lactic acid bacteria peptidoglycan on AFB_(1) were compared by high-performance liquid chromatography,and the Lactobacillus reuteri peptidoglycan with the best in vitro adsorption effect was selected for animal experiment.A total of 120 healthy Cherry Valley ducks were allocated into 5 groups with 4 replicates per group and 6 ducks per replicate.The control group was fed a basal diet,the AFB_(1) group was fed the basal diet supplemented with 0.1 mg/kg AFB_(1),and the experimental groups were fed the AFB_(1) group diets supplemented with 0.10%(groupⅠ),0.15%(groupⅡ)and 0.20%(groupⅢ)Lactobacillus reuteri peptidoglycan,respectively.The pre-experimental period lasted for 3 days,and the experimental period lasted for 21 days.The results showed as follows:1)the species,addition amount and their interaction of lactic acid bacteria peptidoglycan could significantly affect the in vitro adsorption rates for AFB_(1)(P<0.05),and the 10.0 mg/mL Lactobacillus reuteri peptidoglycan had the highest in vitro adsorption rates for AFB_(1),which reached 75.29%.2)Compared with the control group,the average daily weight gain and average daily feed intake of the AFB_(1) group were significantly decreased(P<0.05),and the feed to gain ratio was significantly increased(P<0.05);the aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(AKP)activities and malondialdehyde(MDA)content in plasma were significantly increased(P<0.05),the liver,spleen,thymus and bursa of Fabricius indexes were si

关 键 词：肽聚糖 乳酸菌 黄曲霉毒素B 1 生长性能 脱毒效果 

分 类 号：S834[农业科学—畜牧学]