FBXW12在胰腺癌组织中的表达及其对胰腺癌细胞侵袭和迁移的影响  

Expression of FBXW12 in pancreatic cancer tissues and its effect on the invasion and migration of pancreatic cancer cells

作　　者：张金明 谢永丰 聂娇[2] 鲁临[2] 杜超 ZHANG Jinming;XIE Yongfeng;NIE Jiao;LU Lin;DU Chao(Graduate Training Base of Linyi People’s Hospital,Jinzhou Medical University,Linyi 276000,Shandong,China;Department of Gastroenterology,Linyi People’s Hospital,Linyi 276000,Shandong,China)

机构地区：[1]锦州医科大学临沂市人民医院研究生培养基地,山东临沂276000 [2]临沂市人民医院消化内科,山东临沂276000

出　　处：《中国现代医生》2024年第21期80-85,共6页China Modern Doctor

摘　　要：目的探讨FBXW12在胰腺癌组织中的表达及其对胰腺癌细胞侵袭和迁移的影响。方法采用GEPIA 2数据库分析FBXW12在胰腺癌组织及癌旁正常组织中的表达差异;收集2016年6月至2022年12月于临沂市人民医院行根治术治疗的31例胰腺癌患者的临床资料,对术中切除的癌组织及癌旁正常组织行免疫组化染色检测FBXW12的表达,并随访患者的生存预后情况。利用蛋白质印迹(Western blot)与聚合酶链反应(polymerase chain reaction,PCR)研究胰腺癌细胞株内FBXW12蛋白的表达,并借助Transwell细胞侵袭试验及划痕试验评估FBXW12对胰腺癌细胞侵袭和迁移能力的影响。结果在癌旁正常组织中的FBXW12mRNA表达水平显著高于胰腺癌组织(P<0.05);胰腺癌组织的FBXW12蛋白阳性表达率为75.19%(23/31),癌旁正常组织的FBXW12蛋白阳性表达率为93.55%(29/31),FBXW12在胰腺癌及癌旁正常组织中的表达差异有统计学意义(P<0.05),且胰腺癌中FBXW12表达量与淋巴结转移密切相关(P<0.05),FBXW12低表达组患者预后更差(P<0.05);应用FBXW12过表达质粒转染后,胰腺癌细胞的侵袭和迁移能力均出现显著下降。结论FBXW12在胰腺癌中低表达且与胰腺癌的发生、发展相关。Objective To investigate the expression of FBXW12 in pancreatic cancer and elucidate its impact on cancer cell migration and invasion.Methods The present study utilized the GEPIA 2 database to analyze the differential expression of FBXW12 between pancreatic cancer tissues and normal tissues.Clinical data from 31 pancreatic cancer patients who underwent radical resection at Linyi People’s Hospital from June 2016 to December 2022 were collected.Immunohistochemical staining was conducted to assess FBXW12 expression in both cancerous and adjacent normal tissues obtained during surgery,with subsequent follow-up for survival prognosis.Western blot and polymerase chain reaction(PCR)techniques were employed to determine FBXW12 protein expression levels in pancreatic cancer cell lines.The impact of FBXW12 on cancer cell invasion and migration was evaluated using Transwell cell invasion and scratch test.Results The results from the analysis of the GEPIA 2 database revealed a significant downregulation of FBXW12 mRNA expression in pancreatic cancer tissues compared to normal pancreatic tissues(P<0.05).Immunohistochemical analysis demonstrated a positive expression rate of FBXW12 protein in pancreatic cancer tissues at 75.19%(23/31),whereas adjacent normal tissues exhibited a higher positive expression rate at 93.55%(29/31),indicating a statistically significant difference in FBXW12 expression between pancreatic cancer and adjacent normal tissues(P<0.05).Additionally,the expression level of FBXW12 in pancreatic cancer tissues was found to be closely associated with lymph node metastasis(P<0.05),patients with low expression of FBXW12 have a worse prognosis(P<0.05).Furthermore,transfection with FBXW12 overexpression plasmid resulted in a significant decrease in the invasion and migration abilities of pancreatic cancer cells.Conclusion FBXW12 is low expressed in pancreatic cancer and is associated with the occurrence and development of pancreatic cancer.

关 键 词：FBXW12 胰腺癌 预后 侵袭 迁移 

分 类 号：R576[医药卫生—消化系统]