线粒体DNA含量及10398位点在健康及宫颈癌人群中的分析  

Analysis of mitochondrial DNA content and site 10398 in healthy and cervical cancer populations

作　　者：丰大利[1] 张丽瑾 黄华忠 FENG Dali;ZHANG Lijin;HUANG Huazhong(Department of Oncology,the Second People’s Hospital Affiliated to China Three Gorges University,Yichang Second People’s Hospital,Yichang 443000,Hubei,China;Department of Pharmacy,Traditional Chinese Medicine Hospital of China Three Gorges University,Yichang City Hospital of Traditional Chinese Medicine,Yichang 443000,Hubei,China)

机构地区：[1]三峡大学附属第二人民医院宜昌市第二人民医院肿瘤科,湖北宜昌443000 [2]三峡大学中医医院宜昌市中医医院药剂科,湖北宜昌443000

出　　处：《中国现代医生》2024年第21期86-89,共4页China Modern Doctor

摘　　要：目的探讨血清中线粒体DNA(mitochondrial DNA,mtDNA)含量和10398位点突变情况检测的临床意义。方法收集2020年1月至12月在宜昌市第二人民医院体检的314名健康女性纳入健康体检组,同时收集2020年1月至2023年12月在宜昌市第二人民医院就诊的82例宫颈癌患者纳入宫颈癌组。检测两组纳入者的血清样本mtDNA含量及10398位点基因型,并比较两组纳入者的指标差异。结果宫颈癌组患者的mtDNA含量高于健康体检组,差异有统计学意义(P<0.05);两组纳入者在10398位点基因型差异无统计学意义(P>0.05)。健康体检组中≥45岁的女性mtDNA含量更高,差异有统计学意义(P<0.05);mtDNA在不同10398位点的分布差异无统计学意义(P>0.05)。宫颈癌组中≥45岁的患者mtDNA含量更高,差异有统计学意义(P<0.05);不同临床分期、不同病理类型患者的mtDNA含量差异无统计学意义(P>0.05),mtDNA在不同10398位点中的含量差异无统计学意义(P>0.05)。结论mtDNA含量检测对健康体检人群与宫颈癌人群均有一定的意义,两组纳入者的mtDNA含量均与年龄相关,均随着年龄增长而升高;宫颈癌患者的mtDNA含量明显高于健康体检者;10398位点的血清mtDNA含量检测对两组人群的意义不大;宫颈癌人群的mtDNA含量及10398位点分布与病理、临床分期均无关。mtDNA含量检测可考虑作为宫颈癌诊断的血清标志物。Objective To explore the clinical significance of detecting mitochondrial DNA(mtDNA)content and 10398 site mutation in serum.Methods A tatol of 314 healthy women who underwent physical examinations at the Yichang Second People’s Hospital were collected from January to December 2020 as health examination group,and collect 82 cervical cancer patients who received medical treatment at the Second People’s Hospital of Yichang City from January 2020 to December 2023 as cervical cancer group.Detect the mtDNA content and 10398 locus genotype of serum samples from two groups of participants,and compare the differences in indicators between two groups of participants.Results The mtDNA content of cervical cancer patients was significantly higher than that of healthy examination group(P<0.05);There was no statistically significant difference in genotype at locus 10398 between two groups of participants(P>0.05).The mtDNA content of women aged≥45 in health examination group was higher,and the difference was statistically significant(P<0.05);There was no statistically significant difference in the distribution of mtDNA at different 10398 loci(P>0.05).Patients aged≥45 in cervical cancer group have higher mtDNA content,and the difference is statistically significant(P<0.05);There was no statistically significant difference in mtDNA content among patients with different clinical stages and pathological types(P>0.05),and there was no statistically significant difference in mtDNA content at different 10398 loci(P>0.05).Conclusion The detection of mtDNA content has certain significance for both healthy physical examination population and cervical cancer population.The mtDNA content in both groups is related to age and increases with age.The mtDNA content in cervical cancer population is significantly higher than that in normal physical examination population.The detection of mtDNA at 10398 site in serum has little significance for both groups.The mtDNA content and 10398 site distribution in cervical cancer population are not

关 键 词：线粒体DNA 10398位点突变 宫颈癌 健康人群 

分 类 号：R733[医药卫生—肿瘤]