基于响应面设计优化姬松茸多糖的提取工艺  

Optimization of Agaricus Blazei Polysaccharide Extraction Process Based on Response Surface Design

作　　者：徐清 陈荣林 吴倩媛 王英豪[1] 林建英 XU Qing;CHEN Ronglin;WU Qianyuan;WANG Yinghao;LIN Jianying(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350001,China;Fuzhou Dongxing Biotechnology Co.,Ltd,Fuzhou 350001,China)

机构地区：[1]福建中医药大学药学院,福建福州350001 [2]福州东星生物技术有限公司,福建福州350001

出　　处：《中国民族民间医药》2024年第14期48-55,共8页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:运用响应面设计优化姬松茸多糖的提取工艺。方法:以超声波提取与复合酶酶解法提取姬松茸中的多糖成分,以复合酶添加量、pH值、超声温度、提取时间为考察因素,在单因素实验的基础上,运用响应面设计优化姬松茸多糖的提取工艺。结果:最佳提取工艺条件为复合酶添加量0.03 g、pH6.5、超声温度70℃、提取时间20 min,此条件下姬松茸多糖的得率为10.67%,与预测值11.06%接近。结论:优选出的提取工艺稳定可行,有助于姬松茸的合理运用,为姬松茸多糖有效提取和科学开发提供了参考。Objectives The aim of this project is to optimise the extraction process of Agaricus blazei polysaccharides by using Box-Benhnken.Methods The polysaccharide components in Agaricus blazei were extracted by ultrasonic wave extraction and complex enzyme hydrolysis,and the complex enzyme addition,pH value,ultrasonic temperature and extraction time were investigated,Box-Benhnken was used to optimize the extraction process of Agaricus blazei polysaccharide.Results The optimum extraction process conditions were 0.03g of complex enzyme addition,pH 6.5,ultrasonic temperature of 70°C and extraction time of 20min.The yield of Agaricus blazei polysaccharide under these conditions was 10.67%,which was close to the predicted value of 11.06%.Conclusion The extraction process is stable and feasible,promoting the rational use of Agaricus blazei,and providing a reference for the effective extraction and scientific development of Agaricus blazei polysaccharides.

关 键 词：姬松茸 超声波 酶提法 响应面设计 多糖提取 

分 类 号：R284.2[医药卫生—中药学]