外周血NLRP3炎性小体-Caspase-1-IL-1β信号轴与肺癌相关性分析  

Correlation analysis of peripheral blood NLRP3 inflammasome-caspase-1-IL-1βsignal axis and lung cancer

作　　者：徐兆云 王蕾[1,3] 李世宝 XU Zhaoyun;WANG Lei;LI Shibao(School of Medical Technology,Xuzhou Medical University,Xuzhou 221000;Blood Transfusion Department,Ganyu District People’s Hospital,Lianyungang 222000;Central Laboratory of Hospital,Lianyungang Clinical Medical College of Nanjing Medical University(Lianyungang First People’s Hospital),Lianyungang 222000,China)

机构地区：[1]徐州医科大学医学技术学院,江苏徐州221000 [2]连云港市赣榆区人民医院输血科,江苏连云港222000 [3]南京医科大学连云港临床医学院(连云港市第一人民医院)中心实验室,江苏连云港222000

出　　处：《南京医科大学学报（自然科学版）》2024年第8期1134-1139,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：徐州市医学重点人才(XWRCHT20220065);江苏省预防医学课题(Yl2023029)。

摘　　要：目的:分析外周血核苷酸结合寡聚化结构域样受体蛋白3(nucleotide binding oligomerization domain like receptor protein 3,NLRP3)-半胱氨酸蛋白酶1(cysteine-aspartic acid protease 1,Caspase-1)-白细胞介素(interleukin,IL)-1β信号轴与肺癌的相关性。方法:选取2021年6月—2023年8月江苏省连云港市赣榆区人民医院收治的肺癌住院患者72例初诊患者(肺癌组)和72例健康体检者(健康对照组)。比较两组外周血单核细胞、NLRP3、Caspase-1、IL-1β水平及肺功能[1秒用力呼气量(forced expiratory volume in one second,FEV_(1))、用力肺活量(forced vital capacity,FVC)、FEV_(1)/FVC],并分析肺癌组外周血中NLRP3、IL-1β水平与FEV_(1)、FVC、FEV_(1)/FVC的相关性。结果:肺癌组患者FEV_(1)、FVC、FEV_(1)/FVC水平均显著低于健康对照组,差异有统计学意义(P均<0.05);外周血中Nlrp3、Caspase-1、Il-1βmRNA相对表达水平和Caspase-1和IL-1β分泌水平均高于健康对照组,差异有统计学意义(P均<0.05);Spearman相关分析显示,肺癌组患者外周血单核细胞Nlrp3基因mRNA相对表达量与FEV_(1)、FEV_(1)/FVC水平呈负相关(P<0.05);而外周血IL-1β分泌水平与FEV_(1)/FVC水平呈正相关(P<0.05)。结论:外周血单核细胞中NLRP3 mRNA相对表达水平及外周血IL-1β分泌水平与肺癌进展呈负相关,可反映肺癌进展程度,对肺癌的筛查和诊断具有潜在的参考价值。Objective:To analyze the correlation between peripheral blood nucleotide binding oligomerization domain like receptor protein 3(NLRP3)inflammasome-cysteine protease 1(Caspase-1)-interleukin 1β(IL-1β)signaling axis and lung cancer.Methods:From June 2021 to August 2023,72 patients with lung cancer in our hospital(lung cancer group)and 72 healthy subjects(healthy control group)were selected.The levels of NLRP3,Caspase-1,IL-1βin peripheral blood and peripheral blood of lung cancer control and healthy group subjects were compared and analyzed.The levels of pulmonary function[forced expiratory volume in one second(FEV_(1)),forced vital capacity(FVC),and ratio of forced expiratory volume in the first second to forced vital capacity(FEV_(1)/FVC)]were compared.The correlation between peripheral blood NLRP3 and IL-1βlevels and FEV_(1),FVC and FEV_(1)/FVC was analyzed.Results:The levels of FEV_(1),FVC and FEV_(1)/FVC in lung cancer group were significantly lower than those in healthy control group(P<0.05).The relative mRNA expression levels of Nlrp3,Caspase-1 and IL-1β,as well as the secretion levels of Caspase-1 and IL-1βin peripheral blood of lung cancer group were significantly higher than those of healthy control group(P<0.05).Spearman correlation analysis showed that Nlrp3 gene mRNA relative expression levels in peripheral blood were significantly negatively correlated with FEV_(1)/FVC in lung cancer group(P<0.05).The peripheral blood IL-1βsecretion levels were significantly positively correlated with FEV_(1)and FEV_(1)/FVC in lung cancer group(P<0.05).Conclusion:Nlrp3 gene mRNA relative expression level in peripheral blood monocytes and peripheral blood IL-1βsecretion level are positively correlated with the progression of lung cancer and can reflect the progression of lung cancer to a certain extent,which has potential reference value for the screening and diagnosis of lung cancer.

关 键 词：NLRP3炎性小体 半胱氨酸蛋白酶1 白细胞介素1Β 肺癌 相关性分析 

分 类 号：R734.2[医药卫生—肿瘤]