典型有机磷酸酯阻燃剂暴露下米氏凯伦藻生长及其抗氧化响应研究  

Growth and antioxidant responses of Karenia mikimotoi under exposure to typical organophosphate flame retardants

作　　者：张虹[1,2] 周寒琳 王涛 马帅[1,2] 郭杭婷 张勇[1,2] 韩永和 ZHANG Hong;ZHOU Hanlin;WANG Tao;MA Shuai;GUO Hangting;ZHANG Yong;HAN Yonghe(College of Environmental and Resource Sciences,College of Carbon Neutral Modem Industry,Fujian Normal University,Fuzhou 350117,China;Fujian Key Laboratory of Pollution Control and Resource Reuse,Fuzhou 350117,China)

机构地区：[1]福建师范大学环境与资源学院,碳中和现代产业学院,福建福州350117 [2]福建省污染控制与资源循环利用重点实验室,福建福州350117

出　　处：《海洋环境科学》2024年第4期649-656,共8页Marine Environmental Science

基　　金：福建省中青年教师教育科研项目(JAT190081);福建师范大学高层次引进人才启动经费项目(Y0720304X13)。

摘　　要：有机磷酸酯阻燃剂是一类对海洋微藻具有显著毒性作用的典型新污染物。本文选取两种典型结构的有机磷酸酯阻燃剂磷酸三(2-氯乙基)酯(TCEP)和磷酸三苯酯(TPhP),研究不同浓度(0.5μg/L、5μg/L、50μg/L、500μg/L和5000μg/L)阻燃剂暴露对米氏凯伦藻(Karenia mikimotoi)生长及其抗氧化特征的影响。结果表明,TCEP和TPhP暴露均对米氏凯伦藻的生长产生明显的抑制作用。在暴露第6 d时,TCEP和TPhP对米氏凯伦藻细胞密度的平均抑制率分别达到36.39%和36.69%。藻细胞内可溶性蛋白含量、超氧化物歧化酶(superoxidase dismutase,SOD)和过氧化氢酶(catalase,CAT)活性均随TCEP和TPhP暴露浓度的增加呈现先增加后减少的趋势。其中,藻细胞内可溶性蛋白含量和SOD活性对TCEP胁迫的响应变化较敏感,而CAT活性则对TPhP胁迫的响应变化更敏感。同时,藻细胞内丙二醛(malondialdehyde,MDA)含量随TCEP和TPhP暴露浓度的增加而增加,呈现明显的剂量—效应关系。高浓度(500μg/L和5000μg/L)TCEP暴露下,藻细胞的MDA含量均显著高于对照组(p<0.05),而MDA含量仅在5000μg/L TPhP浓度组显著高于对照组(p<0.05)。研究结果可为评估有机磷酸酯阻燃剂的生态效应提供理论依据。Organophosphate flame retardants(OPFRs)are a class of typical emerging contaminants with significant toxicity to marine microalgae.In this study,the effects of growth and antioxidant characteristics of Karenia mikimotoi under exposure to different concentrations(0.5,5,50,500 and 5000μg/L)of two typical OPFRs[tris(2-chloroethyl)phosphate(TCEP)and triphenyl phosphate(TPhP)]were studied.The results showed that the growth of K.mikimotoi were significantly inhibited by TCEP and TPhP.The average inhibitory rates of TCEP and TPhP on growth of algal cells on the sixth day of exposure were 36.39%and 36.69%,respectively.The soluble protein content and activity of superoxide dismutase(SOD)and catalase(CAT)both increased first and then decreased with the increased exposure concentrations of TCEP and TPhP.Furthermore,the contents of soluble protein and the activity of SOD in algal cells showed higher sensitivity to the stress of TCEP,while the activity of CAT was more sensitive to the stress of TPhP.The content of malondialdehyde(MDA)in algal cells increased with the increase of the TCEP and TPhP concentrations in a dose-dependent manner.And the content of MDA in algal cells treated with 500μg/L and 5000μg/L TCEP concentrations was significantly higher than that in the control(p<0.05),while similar results were found for TPhP only at 5000μg/L(p<0.05).The results can provide a theoretical basis for evaluating the ecological effects of OPFRs.

关 键 词：米氏凯伦藻 磷酸三(2-氯乙基)酯 磷酸三苯酯 氧化应激 

分 类 号：X171.5[环境科学与工程—环境科学]